{
  "abstract": "Precision-cut liver slices (PCLSs) are an ex vivo liver model that allows isolation of extracellular vesicles (EVs), which are nano-sized particles involved in intercellular communication. However, EV recovery from PCLSs is hindered by adhesion to the extracellular matrix and macromolecules such as extracellular DNA. Enzymatic treatments, such as collagenase (COL) and the endonuclease benzonase (BEN), can aid their release, but potential effects on the tissue are unclear. Here, the effect of these enzymes separately, as well as combined, was analyzed to determine their effects on morphology, protein content, and the proteome of the PCLS. The enzymes showed minimal effects on cell viability and protein content after two different incubation times, whereas morphological analysis confirmed collagen degradation by COL and COL(BEN), along with decreased ribosomal and secretory secretory protein levels. BEN decreased the levels of proteins related to protein synthesis and degradation. Increased levels of proteins related to detoxification and energy production were observed upon addition of COL, BEN, and COL(BEN). This study emphasizes the potential and risks for the use of collagenase and benzonase for EV isolation from the PCLS.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 ADGroningen, The Netherlands.",
    "Department of Pharmaceutical Technology and Biopharmacy, Groningen Research Institute of Pharmacy, University of Groningen, 9713 AVGroningen, The Netherlands.",
    "Department of Metabolic Health Research, The Netherlands Organization for Applied Scientific Research (TNO), Leiden2333 BE, The Netherlands.",
    "Drug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, LeuvenB-3000, Belgium.",
    "BioNotus CommV, Niel2845, Belgium.",
    "Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, 9713 GZGroningen, The Netherlands.",
    "Department of Pediatrics, University of Groningen, University Medical Center Groningen, 9713 AVGroningen, The Netherlands."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Janine Stam",
      "orcid": "0000-0002-7157-3450"
    },
    {
      "affs": [
        1
      ],
      "name": "Peter Olinga"
    },
    {
      "affs": [
        2
      ],
      "name": "Aswin Menke"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Pieter Annaert",
      "orcid": "0000-0003-3525-7351"
    },
    {
      "affs": [
        5
      ],
      "name": "Klaas Nico Faber"
    },
    {
      "affs": [
        0
      ],
      "name": "Peter L Horvatovich",
      "orcid": "0000-0003-2218-1140"
    },
    {
      "affs": [
        0
      ],
      "name": "Rainer Bischoff",
      "orcid": "0000-0001-9849-0121"
    },
    {
      "affs": [
        6
      ],
      "name": "Justina C Wolters",
      "orcid": "0000-0003-0066-3720"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5pkyhxwv7mfxa.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.jproteome.5c01232",
  "fulltext_url": "https://europepmc.org/articles/PMC13640514",
  "id": "5pkyhxwv7mfxa",
  "issns": [
    "1535-3893",
    "1535-3907"
  ],
  "landing_url": "https://europepmc.org/article/MED/42918134",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7213473411",
  "pdf_url": "https://europepmc.org/articles/PMC13640514?pdf=render",
  "pmcid": "PMC13640514",
  "pmid": "42918134",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5pkyhxwv7mfxa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42918134",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42918134"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Toxicological Exploration of Collagenase and Benzonase on Rat Precision-Cut Liver Slices.",
  "title_text": "Toxicological Exploration of Collagenase and Benzonase on Rat Precision-Cut Liver Slices.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/5pkyhxwv7mfxa",
  "venue": "Journal of proteome research",
  "versions": []
}