{
  "abstract": "In t(8;21) acute myeloid leukemia (AML), the RUNX1 DNA binding domain is fused to RUNX1T1 (ETO) producing the RUNX1-ETO fusion protein. We previously showed that t(8;21) leukemic stem cells aberrantly activate endothelial signaling pathways to initiate blast cell proliferation. Here, we employed a human embryonic stem cell (ESC) line carrying an inducible RUNX1-ETO transgene to determine whether endothelial signaling pathway activation depends on disease progression or is directly induced by RUNX1-ETO. Single-cell analyses demonstrate that RUNX1-ETO reprograms ESC-derived myeloid progenitors towards endothelial cells. We show (1) that endothelial reprogramming is RUNX1-ETO concentration-dependent and irreversible, (2) at similar RUNX1-ETO and RUNX1 expression levels, a sub-population of blood progenitors can escape reprogramming, and (3) keeps proliferating when co-cultured with endothelial cells. Our experiments highlight the role of cellular interactions in the maintenance of pre-leukemic cells and provide important insights into the earliest stages of genomic reprogramming by oncogenic transcription factors.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Katerina Terolli"
    },
    {
      "name": "Floyd Hassenrueck"
    },
    {
      "name": "Alexander Maytum",
      "orcid": "0009-0003-2341-7239"
    },
    {
      "name": "Peter Keane"
    },
    {
      "name": "Lea Flippe"
    },
    {
      "name": "Sophie G. Kellaway"
    },
    {
      "name": "Merel van der Meulen",
      "orcid": "0000-0002-0001-4408"
    },
    {
      "name": "Olaf T. Heidenreich",
      "orcid": "0000-0001-5404-6483"
    },
    {
      "name": "Edouard G. Stanley"
    },
    {
      "name": "Elizabeth S. Ng"
    },
    {
      "name": "Andrew G. Elefanty"
    },
    {
      "name": "Constanze Bonifer",
      "orcid": "0000-0002-4267-0825"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5psf5q71x67g7.bib",
  "cited_by": 0,
  "doi": "10.1016/j.celrep.2026.118157",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714367/",
  "id": "5psf5q71x67g7",
  "issns": [
    "2211-1247"
  ],
  "landing_url": "https://linkinghub.elsevier.com/retrieve/pii/S2211124726012362",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2720|Acute Myeloid Leukemia Research"
    ]
  ],
  "openalex": "W7222950130",
  "pdf_url": null,
  "pmcid": "PMC13714367",
  "pmid": "42895851",
  "published": "2026-10-01",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/5psf5q71x67g7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:34Z",
      "native_id": "10.1016/j.celrep.2026.118157",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2211124726012362"
    },
    {
      "first_seen": "2026-11-01T12:21:52Z",
      "native_id": "PMC13714367",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.celrep.2026.118157"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2720|Acute Myeloid Leukemia Research",
      "subfield": "general"
    }
  ],
  "title": "RUNX1-ETO oncoprotein levels determine the balance between endothelial reprogramming and pre-leukemic growth in a human ES cell model of AML",
  "title_text": "RUNX1-ETO oncoprotein levels determine the balance between endothelial reprogramming and pre-leukemic growth in a human ES cell model of AML",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:52Z",
  "url": "https://research.draconitic.com/paper/5psf5q71x67g7",
  "venue": "Cell Reports",
  "versions": []
}