{
  "abstract": "Establishment of the blood-brain barrier (BBB) and blood-cerebrospinal fluid (CSF) barrier requires precise coordination between diverse cell types to protect and nourish the brain. Here, we identify developmentally programmed p21 + senescent cells that exhibit divergent senescence-associated features across these two brain interfaces in mice. In the choroid plexus (ChP), epithelial cells adopt a lifelong, non-inflammatory senescent state associated with CSF production and blood-CSF barrier integrity. In contrast, vascular endothelial cells and brain-resident macrophages transiently exhibit pro-inflammatory senescence profiles during brain vascularization, with reciprocal signaling linked to angiogenic patterning and extracellular matrix assembly. The ablation of p21 + cells during mid-gestation disrupts brain vascular patterning and ChP integrity, which results in hemorrhage, impaired CSF production, and ventricular collapse. These findings indicate that embryonic senescent cells adopt divergent transient and long-lived states that support brain-barrier formation and homeostasis, thus reframing the prevailing view of persistent senescence beyond solely a pathological state.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurobiology, University of California, San Diego, La Jolla, CA, USA",
    "Department of Orthopedic Surgery, University of California, San Diego, La Jolla, CA, USA",
    "Department of Neurobiology, University of California, San Diego, La Jolla, CA, USA; Department of Molecular Biology, University of California, San Diego, La Jolla, CA, USA. Electronic address: hmeharena@ucsd.edu"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lauren Ashley Watson"
    },
    {
      "affs": [
        0
      ],
      "name": "Zoe Adelsheim",
      "orcid": "0009-0005-9990-8792"
    },
    {
      "affs": [
        0
      ],
      "name": "Mackenzie J. Carter"
    },
    {
      "affs": [
        0
      ],
      "name": "Grace T. Carter",
      "orcid": "0000-0001-5514-4237"
    },
    {
      "affs": [
        0
      ],
      "name": "Karen L. Jimenez-Reyes"
    },
    {
      "affs": [
        0
      ],
      "name": "Huijie Du"
    },
    {
      "affs": [
        0
      ],
      "name": "Ziqing Zhu",
      "orcid": "0000-0003-2142-1149"
    },
    {
      "affs": [
        1
      ],
      "name": "David B. Berry",
      "orcid": "0000-0001-8275-8322"
    },
    {
      "affs": [
        0
      ],
      "name": "Mia C. Paredez"
    },
    {
      "affs": [
        0
      ],
      "name": "Rania H. Palaniappan"
    },
    {
      "affs": [
        0
      ],
      "name": "John M. Augustine"
    },
    {
      "affs": [
        2
      ],
      "name": "Hiruy S. Meharena",
      "orcid": "0000-0002-0030-2223"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5pxx059nntqg8.bib",
  "cited_by": 5,
  "doi": "10.1016/j.cell.2026.05.022",
  "fulltext_url": null,
  "id": "5pxx059nntqg8",
  "issns": [
    "0092-8674",
    "1097-4172"
  ],
  "landing_url": "https://doi.org/10.1016/j.cell.2026.05.022",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2808|Barrier Structure and Function Studies"
    ]
  ],
  "openalex": "W7164137446",
  "pdf_url": null,
  "pmcid": "PMC13309908",
  "pmid": "42269604",
  "published": "2026-06-10",
  "publisher": "Cell Press",
  "ris": "https://research.draconitic.com/paper/5pxx059nntqg8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:53Z",
      "native_id": "W7164137446",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.cell.2026.05.022"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2808|Barrier Structure and Function Studies",
      "subfield": "neurology"
    }
  ],
  "title": "Persistent and transient senescent cells contribute to brain-barrier development",
  "title_text": "Persistent and transient senescent cells contribute to brain-barrier development",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:14Z",
  "url": "https://research.draconitic.com/paper/5pxx059nntqg8",
  "venue": "Cell",
  "versions": []
}