{
  "abstract": "The bacterium Chlamydia trachomatis causes three distinct conditions – urogenital chlamydia, the eye disease trachoma, and lymphogranuloma venereum. Use of serological testing for exposure to C. trachomatis for evaluating ocular disease risk factors and population-based surveillance has expanded over the past decade. We developed chimeric monoclonal antibodies to the C. trachomatis antigen Pgp3 as standardized reagents for Pgp3 serological assays. Here we use two anti-Pgp3 monoclonals (1G1 and 1C3) to conduct an interlaboratory comparison of Pgp3 Luminex-based multiplex bead serological assays in 4 different laboratories (each with their own protocol) with 5 operators using 3 different instrument types. Antibodies spotted onto filter paper were stable for two weeks at room temperature. Accuracy and precision were evaluated at 3 concentrations for each monoclonal (high, medium, and low). Accuracy within 20% was achieved for 11/21 conditions for 1G1 and 18/21 conditions for 1C3; the high concentration resulted in the poorest accuracy. Precision was within 10% for all low and medium concentrations and was above 10% for several conditions at the high concentration. The fluorescence values differed between instruments but showed no difference when extrapolated to concentrations using a standard curve. These data suggest that 1C3 may be the more reliable standard reagent and that the Pgp3 multiplex assays are highly robust when comparing different instruments and assay protocols.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Parasitic Diseases and Malaria, National Center for Zoonotic, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America",
    "Department of Immunology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom",
    "Infectious Disease Epidemiology and Analytics Unit, Department of Global Health, Institut Pasteur, Université Paris-Cité, Paris, France",
    "Synergy America, Inc., Atlanta, Georgia, United States of America",
    "Eastern and Southern Africa Centre of International Parasite Control, Kenya Medical Research Institute, Nairobi, Kenya"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sarah Gwyn"
    },
    {
      "affs": [
        0
      ],
      "name": "Karana Wickens",
      "orcid": "0000-0003-1437-4321"
    },
    {
      "affs": [
        1
      ],
      "name": "Helena Brazal Monzo"
    },
    {
      "affs": [
        1
      ],
      "name": "Chris Drakeley"
    },
    {
      "affs": [
        2
      ],
      "name": "Stephane Pelleau",
      "orcid": "0000-0002-7070-2177"
    },
    {
      "affs": [
        2
      ],
      "name": "Michael White",
      "orcid": "0000-0002-7472-4138"
    },
    {
      "affs": [
        3
      ],
      "name": "Zachary Reynolds"
    },
    {
      "affs": [
        4
      ],
      "name": "Henry Kanyi"
    },
    {
      "affs": [
        4
      ],
      "name": "Richelle W. Kihoro",
      "orcid": "0000-0003-2512-1645"
    },
    {
      "affs": [
        4
      ],
      "name": "Henry Emisiko",
      "orcid": "0009-0001-5507-9583"
    },
    {
      "affs": [
        4
      ],
      "name": "Sammy M. Njenga",
      "orcid": "0000-0001-6309-476X"
    },
    {
      "affs": [
        0
      ],
      "name": "Diana L. Martin",
      "orcid": "0000-0001-9417-5562"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5q4q6xjd5tg4k.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pgph.0007564",
  "fulltext_url": "https://journals.plos.org/globalpublichealth/article?id=10.1371/journal.pgph.0007564",
  "id": "5q4q6xjd5tg4k",
  "issns": [
    "2767-3375"
  ],
  "landing_url": "https://journals.plos.org/globalpublichealth/article?id=10.1371/journal.pgph.0007564",
  "language": "en",
  "licence": "CC0-1.0",
  "licence_label": "CC0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/publicdomain/zero/1.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Antibodies"
    ],
    [
      "heading",
      "Azides"
    ],
    [
      "heading",
      "Bacteria"
    ],
    [
      "heading",
      "Bacterial Diseases"
    ],
    [
      "heading",
      "Bacterial Pathogens"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Chemical Compounds"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Chlamydia"
    ],
    [
      "heading",
      "Chlamydia Infection"
    ],
    [
      "heading",
      "Chlamydia Trachomatis"
    ],
    [
      "heading",
      "Cloning"
    ],
    [
      "heading",
      "Enzyme-Linked Immunoassays"
    ],
    [
      "heading",
      "Eye Diseases"
    ],
    [
      "heading",
      "Immune Physiology"
    ],
    [
      "heading",
      "Immune System Proteins"
    ],
    [
      "heading",
      "Immunoassays"
    ],
    [
      "heading",
      "Immunologic Techniques"
    ],
    [
      "heading",
      "Immunology"
    ],
    [
      "heading",
      "Infectious Diseases"
    ],
    [
      "heading",
      "Medical Conditions"
    ],
    [
      "heading",
      "Medical Microbiology"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Microbial Pathogens"
    ],
    [
      "heading",
      "Microbiology"
    ],
    [
      "heading",
      "Molecular Biology"
    ],
    [
      "heading",
      "Molecular Biology Techniques"
    ],
    [
      "heading",
      "Monoclonal Antibodies"
    ],
    [
      "heading",
      "Neglected Tropical Diseases"
    ],
    [
      "heading",
      "Ophthalmology"
    ],
    [
      "heading",
      "Organisms"
    ],
    [
      "heading",
      "Pathogens"
    ],
    [
      "heading",
      "Pathology and Laboratory Medicine"
    ],
    [
      "heading",
      "Physical Sciences"
    ],
    [
      "heading",
      "Physiology"
    ],
    [
      "heading",
      "Proteins"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "heading",
      "Research and Analysis Methods"
    ],
    [
      "heading",
      "Serology"
    ],
    [
      "heading",
      "Sexually Transmitted Diseases"
    ],
    [
      "heading",
      "Sodium Azides"
    ],
    [
      "heading",
      "Trachoma"
    ],
    [
      "heading",
      "Tropical Diseases"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Immune system proteins/Antibodies/Monoclonal antibodies"
    ],
    [
      "plos",
      "/Biology and life sciences/Immunology/Immune system proteins/Antibodies/Monoclonal antibodies"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens/Chlamydia trachomatis"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Cloning"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Bacteria/Chlamydia/Chlamydia trachomatis"
    ],
    [
      "plos",
      "/Biology and life sciences/Physiology/Immune physiology/Antibodies/Monoclonal antibodies"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune system proteins/Antibodies/Monoclonal antibodies"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Eye diseases/Trachoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Infectious diseases/Bacterial diseases/Trachoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Infectious diseases/Sexually transmitted diseases/Chlamydia infection"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Tropical diseases/Neglected tropical diseases/Trachoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Ophthalmology/Eye diseases/Trachoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens/Chlamydia trachomatis"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Serology"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Azides/Sodium azides"
    ],
    [
      "plos",
      "/Research and analysis methods/Immunologic techniques/Immunoassays/Enzyme-linked immunoassays"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Cloning"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://journals.plos.org/globalpublichealth/article/file?id=10.1371/journal.pgph.0007564&type=printable",
  "pmcid": "PMC13730333",
  "pmid": "42976167",
  "published": "2026-11-03",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/5q4q6xjd5tg4k.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:20:43Z",
      "native_id": "10.1371/journal.pgph.0007564",
      "source": "plos",
      "url": "https://journals.plos.org/globalpublichealth/article?id=10.1371/journal.pgph.0007564"
    },
    {
      "first_seen": "2026-11-07T12:22:54Z",
      "native_id": "PMC13730333",
      "source": "pmc",
      "url": "https://doi.org/10.1371/journal.pgph.0007564"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Molecular Biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:immunology",
      "subfield": "immunology"
    },
    {
      "field": "biology",
      "origin": "plos:microbiology",
      "subfield": "microbiology"
    },
    {
      "field": "chemistry",
      "origin": "heading:Chemistry",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "plos:infectious diseases",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Interlaboratory comparison of a multiplex bead assay against the Chlamydia trachomatis antigen Pgp3 using two monoclonal antibody clones",
  "title_text": "Interlaboratory comparison of a multiplex bead assay against the Chlamydia trachomatis antigen Pgp3 using two monoclonal antibody clones",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:22:54Z",
  "url": "https://research.draconitic.com/paper/5q4q6xjd5tg4k",
  "venue": "PLOS Global Public Health",
  "versions": []
}