{
  "abstract": "The extracellular Ca2+ is crucial for the regeneration of skeletal muscles, but there is still uncertainty regarding the channels through which it enters the cells. The purpose of this study is to investigate how the Wnt5a/Ca²⁺ signaling pathway promotes myogenic differentiation by regulating which calcium channels on the cell membrane. Myogenic regulatory factors (MRFs) play an important regulatory role in the occurrence and development of skeletal muscle, while overexpression or knockdown of Wnt5a can affect the expression levels of MRFs. Increased expression of Wnt5a resulted in significant increase of intracellular Ca2+ levels, and knockdown of TRPC3 could partially reverse the effect of Wnt5a. This indicates that in addition to the TRPC3 channel, there are other Ca2+ channels involved in the process of transporting extracellular Ca2+ into the cells. When we treated with verapamil, we found that it could significantly reduce the level of Ca2+ entering the cells, indicating that this unknown Ca2+ channel is a slow calcium channel. Through co-immunoprecipitation, we found that CaV1.1 was abundantly accumulated in the TRPC3 pull-down complex, indicating that this slow calcium channel is CaV1.1, and the expression of CaV1.1 is influenced by TRPC3. In simple terms, the activated TRPC3 activates Cav1.1, inducing a slow and sustained inward flow of extracellular Ca2+, leading to the increase of intracellular levels of Ca2+, and finally promote myogenic differentiation.\n\nSupplementary Information: The online version contains supplementary material available at https://doi.org/10.1186/s13287-026-05218-8.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Rehabilitation Medicine, First Affiliated Hospital of Gannan Medical University, 128 Jinling Road, Zhanggong District, Ganzhou, 341000 Jiangxi China",
    "Ganzhou Key Laboratory of Rehabilitation Medicine, Ganzhou, China",
    "School of Basic Medicine, Gannan Medical University, Ganzhou, China",
    "Ganzhou Intelligent Rehabilitation Technology Innovation Center, Ganzhou, China",
    "First School of Clinical Medicine, Gannan Medical University, Ganzhou, China",
    "Department of Oncology, First Affiliated Hospital of Gannan Medical University, 128 Jinling Road, Zhanggong District, Ganzhou, 341000 Jiangxi China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Mao-yuan Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Jin-guo Cao"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Yan-biao Zhong"
    },
    {
      "affs": [
        0
      ],
      "name": "Yun Luo"
    },
    {
      "affs": [
        2
      ],
      "name": "Fu-hua Xie"
    },
    {
      "affs": [
        0
      ],
      "name": "Mei-xuan Zou"
    },
    {
      "affs": [
        4
      ],
      "name": "Wen-juan Wu"
    },
    {
      "affs": [
        4
      ],
      "name": "Xin-ye Ouyang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jia-ming Yang",
      "orcid": "0000-0003-1043-0904"
    },
    {
      "affs": [
        5
      ],
      "name": "Rui-lian Xie",
      "orcid": "0009-0008-0263-2470"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5r0qwym3kyxck.bib",
  "cited_by": 0,
  "doi": "10.1186/s13287-026-05218-8",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13737741/",
  "id": "5r0qwym3kyxck",
  "issns": [
    "1757-6512"
  ],
  "landing_url": "https://doi.org/10.1186/s13287-026-05218-8",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1312|Muscle Physiology and Disorders"
    ]
  ],
  "openalex": "W7197017452",
  "pdf_url": null,
  "pmcid": "PMC13737741",
  "pmid": "42983592",
  "published": "2026-08-07",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/5r0qwym3kyxck.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:51Z",
      "native_id": "PMC13737741",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s13287-026-05218-8"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Muscle Physiology and Disorders",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Wnt5a/Ca2+ signal promotes myogenic differentiation by mediating a slow and sustained influx of Ca2+",
  "title_text": "Wnt5a/Ca2+ signal promotes myogenic differentiation by mediating a slow and sustained influx of Ca2+",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:51Z",
  "url": "https://research.draconitic.com/paper/5r0qwym3kyxck",
  "venue": "Stem Cell Research & Therapy",
  "versions": []
}