{
  "abstract": "Kinetochores connect centromeres to spindle microtubules, ensuring accurate chromosome segregation. Kinetochore assembly at centromeres depends on deposition of CENP-A, a histone H3 variant. Despite considerable progress, how CENP-A is specifically assembled on native centromeres remains an open question. Centromere-specific assembly of CENP-A occurs spontaneously in a cell-free extract system from the yeast Saccharomyces cerevisiae, but with low efficiency, suggesting that one or more factors are limiting. Here, we show Scm3HJURP, a specific chaperone for the centromeric histone Cse4CENP-A, is one such limiting component. The addition of a complex of Scm3, Cse4 and histone H4 strongly stimulates Cse4 assembly in a centromeric DNA sequence- and dose-dependent manner; in addition, improvements in both Cse4 and kinetochore assembly were more prominent in mitotic versus interphase (G1) extracts. Using the complemented cell-free reconstitution system, we show that distinct Scm3 interfaces with Cse4 and with the Ndc10 subunit of the yeast centromere DNA sequence-recognizing CBF3 complex are essential for centromere-specific Cse4 assembly and cell viability. Thus, recognition of Ndc10 directs Scm3-dependent and locus-specific centromeric histone deposition.",
  "abstract_shown": true,
  "affiliations": [
    "University of California, San Diego"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "J. Cai"
    },
    {
      "name": "Z. Wang"
    },
    {
      "name": "L. Cheng"
    },
    {
      "name": "T. Xu"
    },
    {
      "name": "C. Y. Zhang"
    },
    {
      "name": "S. M. Hinshaw"
    },
    {
      "name": "R. T. Suhandynata"
    },
    {
      "name": "A. Desai"
    },
    {
      "affs": [
        0
      ],
      "name": "H. Zhou"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5rpp4jgj55a45.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.30.763002",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.763002v1.full",
  "id": "5rpp4jgj55a45",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.763002v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.763002v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5rpp4jgj55a45.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:20:40Z",
      "native_id": "10.64898/2026.10.30.763002",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.30.763002v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biochemistry",
      "subfield": "general"
    }
  ],
  "title": "Recognition of the centromere-binding protein Ndc10 by Scm3HJURP directs Cse4CENP-A deposition at native centromeres",
  "title_text": "Recognition of the centromere-binding protein Ndc10 by Scm3HJURP directs Cse4CENP-A deposition at native centromeres",
  "type": "preprint",
  "updated_at": "2026-11-04T00:21:04Z",
  "url": "https://research.draconitic.com/paper/5rpp4jgj55a45",
  "venue": "bioRxiv",
  "versions": []
}