{
  "abstract": "Mouse embryonic fibroblasts (MEFs), which are typically derived from mouse embryos between embryonic days 12.5 and 13.5, serve as a widely used feeder layer for pluripotent stem cell culture. MEFs exhibit a limited proliferative capacity and are typically used within five passages after which they undergo early senescence. However, the repeated preparation of primary MEFs introduces batch-to-batch variability and raises ethical concerns regarding animal use, thereby limiting the reproducibility and scalability of stem cell culture. To overcome these limitations, here we established a nontumorigenic immortalized MEF line (designated as SV40-CMEF) from C57BL/6J mice via SV40 large T antigen (SV40LT) transfection. SV40-CMEFs showed genomic integration, enhanced proliferation, and the capacity to support the self-renewal of R1/E mouse embryonic stem cells (mESC) as feeder cells. They also retained their tri-lineage differentiation potential. Notably, SV40-CMEFs did not form colonies in soft agar or tumors in immunodeficient mice. Transcriptomic profiling revealed substantial global remodeling compared with that in primary MEFs; however, functional gene modules associated with feeder capacity, fibroblast identity, and proliferation were selectively retained, whereas senescence markers were suppressed. This cell line provides a convenient, well-characterized resource for stem cell culture and cell differentiation research.",
  "abstract_shown": true,
  "affiliations": [
    "National Collection of Authenticated Cell Cultures, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China",
    "University of Chinese Academy of Sciences, Beijing, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xin Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Ting Shao"
    },
    {
      "affs": [
        0
      ],
      "name": "Lan Xu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Li Zheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Yue-Lei Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5rrkkze33x28g.bib",
  "cited_by": 0,
  "doi": "10.3389/fcell.2026.1936956",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736106/",
  "id": "5rrkkze33x28g",
  "issns": [
    "2296-634X"
  ],
  "landing_url": "https://doi.org/10.3389/fcell.2026.1936956",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "1312|Pluripotent Stem Cells Research"
    ]
  ],
  "openalex": "W7223183728",
  "pdf_url": "https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2026.1936956/pdf",
  "pmcid": "PMC13736106",
  "pmid": "42991093",
  "published": "2026-10-22",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/5rrkkze33x28g.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:38Z",
      "native_id": "PMC13736106",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fcell.2026.1936956"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Pluripotent Stem Cells Research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Establishment and characterization of immortalized mouse embryonic fibroblasts by SV40 large T antigen",
  "title_text": "Establishment and characterization of immortalized mouse embryonic fibroblasts by SV40 large T antigen",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:38Z",
  "url": "https://research.draconitic.com/paper/5rrkkze33x28g",
  "venue": "Frontiers in Cell and Developmental Biology",
  "versions": []
}