{
  "abstract": "Heat stress disrupts protein homeostasis, yet the thermoregulatory roles of HSP10/CPN10 co-chaperonins in polyploid crops remain unexplored. This study conducted the first genome-wide characterization of the HSP10 gene family in allotetraploid Brassica juncea to identify heat tolerance candidates. Genome-wide identification of BjuHSP10 genes was performed across A and B subgenomes, followed by phylogenetic, gene structure, duplication, synteny, promoter, and structural analyses. Transcriptional responses were evaluated under moderate (28/24 °C) and severe (36/32 °C) heat stress conditions. Ten BjuHSP10 genes were identified, encoding six mitochondrial, three chloroplast-targeted, and one cytoplasmic protein. Mitochondrial members were small (~10.7 kDa) with three-exon structures, whereas chloroplast members were larger (15.5-27.1 kDa) with complex exon-intron organization. Segmental duplication drove family expansion (19 duplicated pairs), with differential retention of Arabidopsis homologs. Promoter regions contained 391 putative cis-regulatory elements associated with stress, hormonal, and developmental regulation. Under heat stress, BjuHSP10-4 and BjuHSP10-5 were induced under both treatments; BjuHSP10-1/3/6/9 responded only to severe stress, while four genes were repressed. Structural modeling confirmed conserved β-rich GroES/CPN10-like folds. These findings identify candidate BjuHSP10 genes, particularly BjuHSP10-4 and BjuHSP10-5, as promising targets for heat-tolerance improvement in mustard, providing a foundation for future breeding and functional studies in polyploid crops.",
  "abstract_shown": true,
  "affiliations": [
    "School of Breeding and Multiplication (Sanya Nanfan Research Institute of Breeding and Multiplication), College of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.",
    "Guangxi Key Laboratory of Agro-Environment and Agric Products Safety, Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning 530004, China.",
    "Key Laboratory of Salt-Tolerant Crops Biotechnology of Hainan Province, Sanya 572025, China.",
    "International Center for Interdisciplinary Research in Sciences (ICIRS), The University of Lahore, Lahore 54000, Pakistan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wajid Saeed",
      "orcid": "0009-0007-8537-3859"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhangrong Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Samavia Mubeen"
    },
    {
      "affs": [
        0
      ],
      "name": "Sana Basharat"
    },
    {
      "affs": [
        1
      ],
      "name": "Qiqi Peng"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Huiping Zhao"
    },
    {
      "affs": [
        3
      ],
      "name": "Muzammal Rehman"
    },
    {
      "affs": [
        1
      ],
      "name": "Yun Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Muhammad Waseem",
      "orcid": "0000-0001-7947-7722"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Pingwu Liu",
      "orcid": "0000-0002-0673-8116"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5rwd127z8x2sn.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198640",
  "fulltext_url": "https://europepmc.org/articles/PMC13664748",
  "id": "5rwd127z8x2sn",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883890",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Heat shock proteins research"
    ]
  ],
  "openalex": "W7214661703",
  "pdf_url": "https://europepmc.org/articles/PMC13664748?pdf=render",
  "pmcid": "PMC13664748",
  "pmid": "42883890",
  "published": "2026-09-27",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5rwd127z8x2sn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883890",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883890"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Heat shock proteins research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Genome-Wide Characterization and Heat Responsive Expression Profiling of the HSP10 Co-Chaperonin Gene Family in Brassica juncea.",
  "title_text": "Genome-Wide Characterization and Heat Responsive Expression Profiling of the HSP10 Co-Chaperonin Gene Family in Brassica juncea.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/5rwd127z8x2sn",
  "venue": "International journal of molecular sciences",
  "versions": []
}