{
  "abstract": "Heat stress alters tricarboxylic acid (TCA) cycle metabolism in broiler chickens; however, quantitative changes in TCA cycle-related metabolites remain poorly understood. This study determined the effects of heat exposure on TCA cycle intermediate (citrate, isocitrate, α-ketoglutarate, succinate, fumarate, and malate) and orotate levels in the liver and breast muscle of broiler chickens. Twelve 14-day-old male broiler chickens were assigned to either a control group (25 ± 1 °C) or a cyclic high ambient temperature group (35 ± 1 °C for 8 h/day) and reared under these conditions for 4 days. Heat exposure increased liver levels of citrate, α-ketoglutarate, fumarate, and malate, while decreasing succinate and orotate. Heat exposure also decreased the mRNA expression of isocitrate dehydrogenase 3A (IDH3A) and oxoglutarate dehydrogenase, key TCA cycle regulatory enzymes, as well as dihydroorotate dehydrogenase and ubiquinol–cytochrome c reductase Rieske iron–sulfur protein 1 (UQCRFS1), which are involved in mitochondrial electron transport. Heat exposure increased breast muscle levels of succinate, fumarate, and malate, while reducing IDH3A and UQCRFS1 mRNA expression. These findings suggest that heat exposure impairs TCA cycle metabolism in broiler chickens, possibly by reducing the utilization of TCA cycle intermediates through impaired electron transfer in mitochondrial complex III.",
  "abstract_shown": true,
  "affiliations": [
    "United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan",
    "Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan",
    "South Kyushu Livestock Veterinary Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.",
    "Graduate School of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ayumi Katafuchi"
    },
    {
      "affs": [
        0
      ],
      "name": "Saki Shimamoto"
    },
    {
      "affs": [
        1
      ],
      "name": "Shozo Tomonaga"
    },
    {
      "affs": [
        2
      ],
      "name": "Miyu Kamimura"
    },
    {
      "affs": [
        3
      ],
      "name": "Shinya Ishihara"
    },
    {
      "affs": [
        0
      ],
      "name": "Akira Ohtsuka"
    },
    {
      "affs": [
        0
      ],
      "name": "Daichi Ijiri"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5s1anrfbg55y0.bib",
  "cited_by": 0,
  "doi": "10.2141/jpsa.2026024",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13696759/",
  "id": "5s1anrfbg55y0",
  "issns": [
    "1346-7395",
    "1349-0486"
  ],
  "landing_url": "https://doi.org/10.2141/jpsa.2026024",
  "language": "en",
  "licence": "CC-BY-NC-SA-4.0",
  "licence_label": "CC BY-NC-SA 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-sa/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Full Paper"
    ],
    [
      "openalex",
      "1103|Effects of Environmental Stressors on Livestock"
    ]
  ],
  "openalex": "W7223963436",
  "pdf_url": "https://www.jstage.jst.go.jp/article/jpsa/63/0/63_2026024/_pdf",
  "pmcid": "PMC13696759",
  "pmid": "42936156",
  "published": "2026-10-28",
  "publisher": "Japan Poultry Science Association",
  "ris": "https://research.draconitic.com/paper/5s1anrfbg55y0.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:01Z",
      "native_id": "PMC13696759",
      "source": "pmc",
      "url": "https://doi.org/10.2141/jpsa.2026024"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Effects of Environmental Stressors on Livestock",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Effects of Cyclic High Ambient Temperature on Tricarboxylic Acid Cycle Intermediates and Orotate Levels in the Liver and Breast Muscle of Broiler Chickens",
  "title_text": "Effects of Cyclic High Ambient Temperature on Tricarboxylic Acid Cycle Intermediates and Orotate Levels in the Liver and Breast Muscle of Broiler Chickens",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:01Z",
  "url": "https://research.draconitic.com/paper/5s1anrfbg55y0",
  "venue": "The Journal of Poultry Science",
  "versions": []
}