{
  "abstract": "The potential of senescent cell clearance to extend healthspan and delay the onset of age-related diseases has driven the development of innovative senotherapeutics. Thus, niosome-based gene therapy has emerged as a promising strategy to overcome the off-target limitations of first-generation senolytics, senomorphics, and senoreverts. In the present study, we investigated two new niosome formulations for genetically modifying primary umbilical cord stroma senescent MSCs (UC-MSCs) in a Palbociclib-induced senescent model, using non-senescent UC-MSCs as a control. These niosomes were formulated by fixing the cationic lipid composition (1,2-di-O-octadecenyl-3 trimethylammonium propane, DOTMA, D) and varying the non-ionic surfactants (polysorbate 20, P20, or polysorbate 80, P80) and the helper lipids (chloroquine, CQ, or cholesterol, CH) (DP20CQ and DP80CH, respectively) at two mole ratios (1:2:1 and 1:2:2 for DP20CQ; 1:2:2 and 1:2:4 for DP80CH). Results on transfection revealed that the DP20CQ formulations outperformed those with DP80CH in both UC-MSCs phenotypes from four different donors. Notably, DP20CQ niosomes prepared at 10/1 and 15/1 DOTMA/DNA ratios achieved the highest transgene expression levels in senescent UC-MSCs, surpassing even the commercial reagent Lipofectamine. Further, DP20CQ formulations exhibited adequate physicochemical properties for transfection and promoted a marked endosomal escape, particularly at the DOTMA/DNA ratio 10/1, without compromising cell viability. These findings underscore the value of niosomes as robust gene delivery vehicles for senescent cells in therapeutic antiaging strategies.",
  "abstract_shown": true,
  "affiliations": [
    "CICA - Centro Interdisciplinar de Química e Bioloxía, Universidade da Coruña, A Coruña, Spain.",
    "Departamento de Bioloxía, Facultade de Ciencias, Universidade da Coruña, A Coruña, Spain.",
    "Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Servizo Galego de Saúde (SERGAS), A Coruña, Spain.",
    "Departamento de Medicina, Facultade de Ciencias da Saúde, Universidade da Coruña (UDC), A Coruña, Spain."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Alba Iglesias-Fente"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Junquera López-Seijas"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "María C Arufe",
      "orcid": "0000-0003-3725-0743"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Juan Antonio Fafián-Labora"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ana Rey-Rico"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5vpe32cvar2mn.bib",
  "cited_by": 0,
  "doi": "10.1080/10717544.2026.2726650",
  "fulltext_url": "https://europepmc.org/articles/PMC13651833",
  "id": "5vpe32cvar2mn",
  "issns": [
    "1071-7544",
    "1521-0464"
  ],
  "landing_url": "https://europepmc.org/article/MED/42845215",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Interference and Gene Delivery"
    ]
  ],
  "openalex": "W7221035153",
  "pdf_url": "https://europepmc.org/articles/PMC13651833?pdf=render",
  "pmcid": "PMC13651833",
  "pmid": "42845215",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5vpe32cvar2mn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42845215",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42845215"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA Interference and Gene Delivery",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Innovative niosome screening for efficient gene delivery in senescent primary umbilical cord stroma-derived MSCs.",
  "title_text": "Innovative niosome screening for efficient gene delivery in senescent primary umbilical cord stroma-derived MSCs.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/5vpe32cvar2mn",
  "venue": "Drug delivery",
  "versions": []
}