{
  "abstract": "Psilocybin produces rapid and lasting therapeutic effects, yet how 5-HT2A receptor activation reshapes brain-wide circuit dynamics during acute drug administration remains poorly understood. Using simultaneous multi-region Neuropixels recordings of 46,360 single units from 35 mice, together with scalp electroencephalography (EEG), pupillometry, and locomotion monitoring, we provide a brain-wide, single-unit and field-potential characterization of psilocybin's acute effects, with pharmacological dissection using the 5-HT2A antagonist ketanserin. Psilocybin selectively reconfigured burst coding, rather than mean firing rate, across cortical, thalamic, and hippocampal circuits: burst firing decreased in hippocampal CA1-CA3 and was bidirectionally modulated in the thalamus, with the reticular nucleus bursting more and first-order geniculate nuclei bursting less. Critically, most of these burst effects were abolished by ketanserin, consistent with at least partial 5-HT2A receptor dependence. These data suggest that the psychedelic state is not simply a matter of how much neurons fire, but of how they fire, pointing to a region-specific, 5-HT2A-associated reconfiguration of burst coding that may underlie the acute phenomenology of the psilocybin experience.",
  "abstract_shown": true,
  "affiliations": [
    "Allen Institute, Brain and Consciousness, Seattle"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "D. Momi"
    },
    {
      "name": "Y. Nahas"
    },
    {
      "name": "D. Wyrick"
    },
    {
      "name": "L. C. Marks"
    },
    {
      "name": "L. D. Claar"
    },
    {
      "name": "R. De Filippo"
    },
    {
      "name": "P. Seyfourian"
    },
    {
      "name": "D. A. Pizzagalli"
    },
    {
      "name": "M. Buice"
    },
    {
      "name": "T. Ott"
    },
    {
      "name": "C. Koch"
    },
    {
      "affs": [
        0
      ],
      "name": "I. Rembado"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5wt299z4xfy91.bib",
  "cited_by": 1,
  "doi": "10.64898/2026.08.14.744865",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.14.744865v2.full",
  "id": "5wt299z4xfy91",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.14.744865v2",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "3203|Psychedelics and Drug Studies"
    ]
  ],
  "openalex": "W7203789443",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.14.744865v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5wt299z4xfy91.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:52Z",
      "native_id": "10.64898/2026.08.14.744865",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.14.744865v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Brain-wide reconfiguration of burst firing by psilocybin reveals 5-HT2A-dependent circuit dynamics",
  "title_text": "Brain-wide reconfiguration of burst firing by psilocybin reveals 5-HT2A-dependent circuit dynamics",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:52Z",
  "url": "https://research.draconitic.com/paper/5wt299z4xfy91",
  "venue": "bioRxiv",
  "versions": []
}