{
  "abstract": "The ability of the rhizosphere to recruit beneficial microorganisms is central to the sustainable management of crops. Here, we investigated, through shotgun metagenomic sequencing and mass spectrometry techniques, the microbial composition, antimicrobial resistance (AMRs), and metabolomic profiles of Piper nigrum cultivated under two support systems: living Gliricidia sepium support (SL/RL) and dead wooden support (SD/RD). Bulk and rhizosphere soils were sequenced to assess taxonomic structure and ARGs, with emphasis on Actinobacteria due to their relevance in antibiotic production. Actinobacteria were highly represented across the samples (~ 35%). Dead-support rhizospheres exhibited a broader ARG repertoire and greater abundance of taxa known to include antibiotic-producing members, whereas living-support rhizospheres showed reduced ARG diversity. Metabolomic analysis further revealed support-dependent physiological responses in the host plant. PLS-DA distinguished the two cultivation systems, and three defense-related flavonoids, such as sakuranetin, isovitexin, and orientin, which were enriched in fruits of plants grown with dead support, indicating activation of stress - and defense - associated metabolic pathways. Their low abundance in living-support plants suggests reduced physiological stress, likely linked to improved nutrient cycling and rhizosphere stability provided by G. sepium. However, the volatile and total phenolic profile did not display significant chemical alterations in the samples. Together, these results suggest that support type influences both microbial functions and plant secondary metabolism. Dead support creates a more stressful ecological context that enriches antimicrobial resistance genes ARGs and defense metabolites, whereas living support promotes a more balanced soil-plant-microbe environment, supporting the use of G. sepium as a sustainable and biologically advantageous cultivation strategy for black pepper.",
  "abstract_shown": true,
  "affiliations": [
    "Socioenvironmental and Water Resources Institute, Federal Rural University of the Amazon - UFRA, Belém, Pará, Brazil.",
    "Laboratory of Simulation and Computational Biology, High Performing Computer Center, Federal University of Pará - UFPA, Belém, Pará, Brazil.",
    "Laboratory of Bioinformatics and Genomics of Microorganisms, Institute of Biological Science, Federal University of Pará - UFPA, Belém, Pará, Brazil.",
    "Institute of Biological Science, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.",
    "Laboratório de Bioprospecção e Inovação Tecnológica de Produtos Naturais da Amazônia, Federal University of Pará - UFPA, Belém, Pará, Brazil.",
    "Amazon Integrated Metabolomics Center, Institute of Exact and Natural Sciences, Federal University of Pará - UFPA, Belém, Brazil.",
    "Laboratório de Bioprospecção e Inovação Tecnológica de Produtos Naturais da Amazônia, Federal University of Pará - UFPA, Belém, Pará, Brazil. joycekellys@ufpa.br.",
    "Socioenvironmental and Water Resources Institute, Federal Rural University of the Amazon - UFRA, Belém, Pará, Brazil. rommelthiago@gmail.com.",
    "Laboratory of Simulation and Computational Biology, High Performing Computer Center, Federal University of Pará - UFPA, Belém, Pará, Brazil. rommelthiago@gmail.com.",
    "Laboratory of Bioinformatics and Genomics of Microorganisms, Institute of Biological Science, Federal University of Pará - UFPA, Belém, Pará, Brazil. rommelthiago@gmail.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Valéria Alves Chaves"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Oscar Victor Cardenas Alegria"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Carlos Willian Dias Dantas"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Victor Benedito Costa Ferreira"
    },
    {
      "affs": [
        1,
        3,
        2
      ],
      "name": "David Tavares Martins"
    },
    {
      "affs": [
        4
      ],
      "name": "Rhodin Clarentz Joseph"
    },
    {
      "affs": [
        4
      ],
      "name": "Laís Sousa Almeida"
    },
    {
      "affs": [
        5
      ],
      "name": "Sandro Duarte de Souza"
    },
    {
      "affs": [
        5
      ],
      "name": "Paulo Wender P Gomes"
    },
    {
      "affs": [
        6
      ],
      "name": "Joyce Kelly R da Silva"
    },
    {
      "affs": [
        7,
        8,
        9
      ],
      "name": "Rommel Thiago Jucá Ramos"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5xxw8x0j6jj5m.bib",
  "cited_by": 0,
  "doi": "10.1007/s11274-026-05278-1",
  "fulltext_url": "https://europepmc.org/articles/PMC13722817",
  "id": "5xxw8x0j6jj5m",
  "issns": [
    "1573-0972",
    "0959-3993"
  ],
  "landing_url": "https://europepmc.org/article/MED/42963482",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1110|Plant-Microbe Interactions and Immunity"
    ]
  ],
  "openalex": "W7224861164",
  "pdf_url": "https://europepmc.org/articles/PMC13722817?pdf=render",
  "pmcid": "PMC13722817",
  "pmid": "42963482",
  "published": "2026-10-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5xxw8x0j6jj5m.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T03:21:21Z",
      "native_id": "MED:42963482",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42963482"
    },
    {
      "first_seen": "2026-11-04T12:21:34Z",
      "native_id": "PMC13722817",
      "source": "pmc",
      "url": "https://doi.org/10.1007/s11274-026-05278-1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant-Microbe Interactions and Immunity",
      "subfield": "plant-biology"
    }
  ],
  "title": "Influence of Gliricidia sepium living support on rhizosphere bacterial recruitment and fruit metabolomics in black pepper (Piper nigrum).",
  "title_text": "Influence of Gliricidia sepium living support on rhizosphere bacterial recruitment and fruit metabolomics in black pepper (Piper nigrum).",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:21:34Z",
  "url": "https://research.draconitic.com/paper/5xxw8x0j6jj5m",
  "venue": "World journal of microbiology & biotechnology",
  "versions": []
}