{
  "abstract": "Objective: Chronic alcohol consumption is one of the leading causes of dilated cardiomyopathy. Alcohol-associated cardiomyopathy (ACM) is characterized by impaired cardiac function and ventricular dilation in individuals with a history of long-term alcohol consumption. Alcohol exerts adverse effects on the myocardium via mitochondrial dysfunction and chronic inflammation. However, the broader transcriptional changes associated with chronic alcohol exposure remain poorly understood.\n\nMethods: A chronic ethanol feeding model was utilized, in which male Wistar rats were fed a Lieber-Decarli ethanol diet containing 6.7% (v/v) ethanol (ET) or an isocaloric control (CONT) diet for 6 weeks. Left ventricular tissues were collected from ET-fed and CONT-fed rats. Differential gene expression was assessed using bulk RNA sequencing, followed by Ingenuity Pathway Analysis (IPA).\n\nResults: Transcriptomic analysis identified 194 upregulated and 199 downregulated genes in the left ventricles of ET-fed rats compared with CONT-fed rats. IPA revealed downregulated pathways related to the circadian clock and interferon signaling in the myocardium of ET-fed rats. In particular, the differentially altered genes included regulators of the circadian clock ( Bmal1, Npas2, Per1 , Per2 ) and interferon signaling ( Ifit2 , Ifit3 , Irf6 , Irf9 ). In contrast, pathways associated with mitochondrial RNA degradation, sheddase signaling, and neutrophil extracellular traps were upregulated in the myocardium following chronic ethanol exposure.\n\nConclusion: Chronic ethanol exposure is associated with dysregulation of circadian rhythm and immune-related signaling pathways in the rat myocardium. These findings highlight molecular pathways that may contribute to the development of ACM and warrant further mechanistic investigation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, University of Nebraska Medical Center",
    "VA Nebraska-Western Iowa Healthcare System",
    "Department of Internal Medicine, Division of Gastroenterology and Hepatology, University of Nebraska Medical Center",
    "Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Weilun Ai"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Carol A. Casey"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Karuna Rasineni"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Viswanathan Saraswathi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5xz6ngj8y0ynx.bib",
  "cited_by": 0,
  "doi": "10.3389/fcvm.2026.1977478",
  "fulltext_url": "https://europepmc.org/articles/PMC13713737",
  "id": "5xz6ngj8y0ynx",
  "issns": [
    "2297-055X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fcvm.2026.1977478/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2734|Alcohol Consumption and Health Effects"
    ]
  ],
  "openalex": "W7221834628",
  "pdf_url": "https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2026.1977478/pdf",
  "pmcid": "PMC13713737",
  "pmid": "42953082",
  "published": "2026-10-14",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/5xz6ngj8y0ynx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "10.3389/fcvm.2026.1977478",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fcvm.2026.1977478/full"
    },
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953082",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953082"
    },
    {
      "first_seen": "2026-10-31T12:24:14Z",
      "native_id": "PMC13713737",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fcvm.2026.1977478"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2297-055X",
      "subfield": "cardiovascular"
    }
  ],
  "title": "Effects of chronic alcohol consumption on gene expression in the rat left ventricle: a transcriptomic analysis",
  "title_text": "Effects of chronic alcohol consumption on gene expression in the rat left ventricle: a transcriptomic analysis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:21Z",
  "url": "https://research.draconitic.com/paper/5xz6ngj8y0ynx",
  "venue": "Frontiers in Cardiovascular Medicine",
  "versions": []
}