{
  "abstract": "In plant genomes, transposable elements (TEs) are silenced by CG and non-CG DNA methylation, with non-CG methylation maintained by CMT chromomethylases and the RNA-directed DNA methylation (RdDM) pathway. In Arabidopsis, linker histone H1 differentially influences these pathways, as loss of H1 causes non-CG hypermethylation at pericentromeric TEs but hypomethylation at euchromatic TEs in gene-rich regions. The molecular basis of this antagonism has remained unclear. Here, we show that GH1-HMGAs, a family of H1-related High Mobility Group proteins, bind AT-rich sequences at euchromatic TEs and prevent RdDM-dependent non-CG methylation by limiting siRNA accumulation. Furthermore, GH1-HMGA and H1 compete for chromatin association at these TEs, as loss of GH1-HMGA triggers ectopic H1 redistribution, while loss of H1 increases GH1-HMGA enrichment, providing a mechanistic explanation for the non-CG hypomethylation observed in h1 mutants. Collectively, these findings reveal how GH1-HMGA proteins specifically restrain RdDM activity at euchromatic TEs and elucidate how the H1/GH1-HMGA balance spatially partitions distinct DNA methylation pathways across the genome.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "Léa Feit"
    },
    {
      "name": "Alejandro Edera"
    },
    {
      "name": "Emmanuel Vanrobays"
    },
    {
      "name": "Lauriane Simon"
    },
    {
      "name": "Delphine Dardalhon-Cuménal"
    },
    {
      "name": "Saad En Naimani"
    },
    {
      "name": "Zdravko J Lorković"
    },
    {
      "name": "Frederic Berger"
    },
    {
      "name": "Fredy Barneche"
    },
    {
      "name": "Christophe Tatout"
    },
    {
      "name": "Leandro Quadrana"
    },
    {
      "name": "Aline V Probst"
    },
    {
      "name": "Simon Amiard"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5y3aera6j26yn.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.08.749845",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.08.749845v1.full",
  "id": "5y3aera6j26yn",
  "issns": [],
  "landing_url": "https://hal.science/hal-05778251v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genetics"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "1.sdv.gen"
    ],
    [
      "hal",
      "2.sdv.bbm.gtp"
    ],
    [
      "hal",
      "2.sdv.gen.gpl"
    ],
    [
      "openalex",
      "1312|Epigenetics and DNA Methylation"
    ]
  ],
  "openalex": "W7212303489",
  "pdf_url": "https://hal.science/hal-05778251/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5y3aera6j26yn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:03Z",
      "native_id": "hal-05778251",
      "source": "hal",
      "url": "https://hal.science/hal-05778251v1"
    },
    {
      "first_seen": "2026-10-30T12:21:15Z",
      "native_id": "10.64898/2026.09.08.749845",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.08.749845v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
    },
    {
      "field": "biology",
      "origin": "biorxiv:genetics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "GH1-HMGA proteins restrict RNA-directed DNA methylation and antagonize linker histone H1 at euchromatic transposable elements",
  "title_text": "GH1-HMGA proteins restrict RNA-directed DNA methylation and antagonize linker histone H1 at euchromatic transposable elements",
  "type": "preprint",
  "updated_at": "2026-10-31T12:20:56Z",
  "url": "https://research.draconitic.com/paper/5y3aera6j26yn",
  "venue": "HAL",
  "versions": []
}