{
  "abstract": "Autologous nerve grafts remain the gold standard for peripheral nerve repair, but donor scarcity, donor-site morbidity and incomplete functional recovery limit their use. Schwann cells (SCs) drive autograft-mediated regeneration, yet their limited availability and expansion impede SC-based therapies. Here, we generated human pluripotent stem cell-derived SCs, motor neuron-enriched spinal cord organoids and nociceptive sensory neuron-enriched dorsal root ganglion organoids, and assembled them into a nerve-like construct termed the Peri-nervoid. Single-cell RNA sequencing revealed context-dependent SC plasticity: axonal co-culture promoted myelination, whereas axonal transection induced a repair phenotype resembling SC dedifferentiation during Wallerian degeneration. Following transplantation into 7-mm sciatic nerve defects in NSG mice, human SCs survived and remyelinated regenerating host axons, while axon-containing grafts enhanced regeneration relative to SC-only grafts. No abnormal proliferation, systemic toxicity or major-organ histopathology was detected. These findings position the Peri-nervoid as a scalable, developmentally inspired platform for developing engineered grafts for peripheral nerve repair.",
  "abstract_shown": true,
  "affiliations": [
    "Beijing Jishuitan Hospital, Capital Medical University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "Y. Guan"
    },
    {
      "name": "R. He"
    },
    {
      "name": "Z. Xiang"
    },
    {
      "name": "Y. Ouyang"
    },
    {
      "name": "H. Liu"
    },
    {
      "name": "S. Zhang"
    },
    {
      "name": "X. Liu"
    },
    {
      "name": "C. Liu"
    },
    {
      "name": "Z. Wang"
    },
    {
      "name": "Y. Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6104etvp95rs2.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.14.750880",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750880v1.full",
  "id": "6104etvp95rs2",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750880v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "2804|Nerve injury and regeneration"
    ]
  ],
  "openalex": "W7213224586",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750880v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6104etvp95rs2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:39Z",
      "native_id": "10.64898/2026.09.14.750880",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750880v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Generation and application of human pluripotent stem cells derived peri-nervoids",
  "title_text": "Generation and application of human pluripotent stem cells derived peri-nervoids",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:39Z",
  "url": "https://research.draconitic.com/paper/6104etvp95rs2",
  "venue": "bioRxiv",
  "versions": []
}