{
  "abstract": "A mixture of lactonic and acidic sophorolipids exerted concentration-dependent cytotoxicity against human breast ductal adenocarcinoma (MCF-7) cells, with an IC₅₀ of 82.54 µg/mL as determined by resazurin assay. Phase-contrast microscopy revealed morphological alterations including cell swelling, loss of adhesion, and impaired membrane integrity. Flow cytometry analysis demonstrated cell cycle arrest in the S phase, mitochondrial membrane depolarization, and a predominance of necrosis-like cell death at higher concentrations. Transcriptional profiling by RT-qPCR identified overexpression of CDKN1A, GADD45A, ERN1, and BBC3, alongside repression of ACTB and GAPDH, indicating activation of DNA damage and stress-response pathways, endoplasmic reticulum stress, structural collapse, and metabolic dysfunction. Collectively, these findings demonstrate that sophorolipids induce mitochondrial and endoplasmic reticulum stress, culminating in homeostatic failure and cell death in MCF-7 cells, positioning these biosurfactants as promising candidates for the development of natural antiproliferative therapies.Supplementary information\n\nThe online version contains supplementary material available at https://doi.org/10.1007/s13205-026-05096-0.",
  "abstract_shown": true,
  "affiliations": [
    "Department of General Biology, State University of Londrina, Londrina, Paraná Brazil.",
    "Department of Biochemistry and Biotechnology, State University of Londrina, Londrina, Paraná Brazil."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Luiz Henrique Santana Martins",
      "orcid": "0009-0000-1375-9436"
    },
    {
      "affs": [
        0
      ],
      "name": "Luan Vitor Alves de Lima",
      "orcid": "0000-0002-0360-2889"
    },
    {
      "affs": [
        0
      ],
      "name": "Matheus Felipe da Silva"
    },
    {
      "affs": [
        1
      ],
      "name": "Niumaique Gonçalves da Silva",
      "orcid": "0000-0001-8068-9466"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Claudia Terkelli de Assis",
      "orcid": "0009-0005-8353-6832"
    },
    {
      "affs": [
        0
      ],
      "name": "Isabella Cristina Oliveira Carvalho"
    },
    {
      "affs": [
        0
      ],
      "name": "Isaura Maria Fuzzinato",
      "orcid": "0009-0001-6422-7912"
    },
    {
      "affs": [
        0
      ],
      "name": "Beatriz Braga Silva",
      "orcid": "0009-0007-1420-1929"
    },
    {
      "affs": [
        0
      ],
      "name": "Dimitri Alves da Costa",
      "orcid": "0009-0001-4653-2892"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Mattheus Pizane de Azevedo Silva",
      "orcid": "0009-0001-4820-8236"
    },
    {
      "affs": [
        1
      ],
      "name": "Maria Antonia Pedrine Colabone Celligoi",
      "orcid": "0000-0003-2217-8937"
    },
    {
      "affs": [
        0
      ],
      "name": "Mario Sérgio Mantovani",
      "orcid": "0000-0001-5268-6508"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/61p0vdvt4wh54.bib",
  "cited_by": 0,
  "doi": "10.1007/s13205-026-05096-0",
  "fulltext_url": "https://europepmc.org/articles/PMC13694592",
  "id": "61p0vdvt4wh54",
  "issns": [
    "2190-572X",
    "2190-5738"
  ],
  "landing_url": "https://europepmc.org/article/MED/42926778",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Natural product bioactivities and synthesis"
    ]
  ],
  "openalex": "W7223391838",
  "pdf_url": "https://europepmc.org/articles/PMC13694592?pdf=render",
  "pmcid": "PMC13694592",
  "pmid": "42926778",
  "published": "2026-10-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/61p0vdvt4wh54.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:18Z",
      "native_id": "MED:42926778",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42926778"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Natural product bioactivities and synthesis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Investigation of the molecular mechanisms of action of sophorolipids in MCF-7 cells: therapeutic potential against breast cancer.",
  "title_text": "Investigation of the molecular mechanisms of action of sophorolipids in MCF-7 cells: therapeutic potential against breast cancer.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:18Z",
  "url": "https://research.draconitic.com/paper/61p0vdvt4wh54",
  "venue": "3 Biotech",
  "versions": []
}