{
  "abstract": "Wildlife disease investigations require diagnostic methods capable of detecting diverse and previously unrecognized pathogens. Metagenomic next-generation sequencing (mNGS) was applied to samples from three Dorcas gazelles (Gazella dorcas osiris) affected by sudden mortality and four European bison (Bison bonasus) with respiratory disease or necrotic balanoposthitis (NBP). No dominant bacterial species was detected in gazelles. In European bison, respiratory disease was associated with Pasteurella multocida, whereas NBP samples were dominated by Porphyromonas levii and other anaerobes. Dorcas gazelle samples contained mainly Retroviridae-related sequences of undetermined pathogenic significance. Parapoxvirus-related annotations were not confirmed by PCR, suggesting non-specific or host-related sequence similarity. Macavirus-related sequences were consistent with previous PCR findings and enabled reconstruction of an approximately 51.1 kb fragment of a divergent gammaherpesvirus genome. These findings demonstrate the value of mNGS for wildlife disease investigation and genomic surveillance, while emphasizing the need for independent validation of metagenomic taxonomic assignments.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Virology and Viral Animal Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.",
    "Department of Evolutionary and Physiological Ecology, Faculty of Biology, University of Białystok, K. Ciołkowskiego 1J, 15-245 Białystok, Poland.",
    "\"Medicor\" Veterinary Service, Zwycięstwa 26B, 15-703 Białystok, Poland.",
    "Zamość Zoological Garden, Szczebrzeska 12, 22-400 Zamość, Poland.",
    "Veterinary Clinic, Jana Kilinskiego 3A, 43-200 Pszczyna, Poland."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wojciech Socha",
      "orcid": "0000-0002-3782-7971"
    },
    {
      "affs": [
        1
      ],
      "name": "Michał K Krzysiak"
    },
    {
      "affs": [
        2
      ],
      "name": "Elżbieta Moniuszko"
    },
    {
      "affs": [
        3
      ],
      "name": "Paweł Kulik"
    },
    {
      "affs": [
        4
      ],
      "name": "Jarosław Tomana"
    },
    {
      "affs": [
        0
      ],
      "name": "Magdalena Larska",
      "orcid": "0000-0003-2069-3011"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/61yyy56bzz1zf.bib",
  "cited_by": 0,
  "doi": "10.3390/v18101118",
  "fulltext_url": "https://europepmc.org/articles/PMC13709774",
  "id": "61yyy56bzz1zf",
  "issns": [
    "1999-4915"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944039",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2739|Zoonotic diseases and public health"
    ]
  ],
  "openalex": "W7221101973",
  "pdf_url": "https://europepmc.org/articles/PMC13709774?pdf=render",
  "pmcid": "PMC13709774",
  "pmid": "42944039",
  "published": "2026-10-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/61yyy56bzz1zf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42944039",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944039"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Zoonotic diseases and public health",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Next-Generation Sequencing in Practical Wildlife Disease Investigation: Metagenomic Insights into European Bison and Gazelle Mortality and Morbidity Events.",
  "title_text": "Next-Generation Sequencing in Practical Wildlife Disease Investigation: Metagenomic Insights into European Bison and Gazelle Mortality and Morbidity Events.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/61yyy56bzz1zf",
  "venue": "Viruses",
  "versions": []
}