{
  "abstract": "Mixed bacterial infections, including Gram-positive and Gram-negative pathogens, remain a major global health concern. Achieving efficient treatment of synergistic polymicrobial infections stills a tremendous clinical challenge due to antibiotic permeability barriers and resistance. Here, we construct a defect-engineered layered double hydroxide (LDH)-based sonosensitizer platform loaded with clindamycin (a-CoW/CLD) for mixed bacterial infection therapy, which not only demonstrates high efficacy against Gram-positive bacteria, but also exploits reactive oxygen species (ROS)-mediated membrane disruption of Gram-negative bacteria to facilitate CLD penetration, thereby achieving broad-spectrum and highly efficient killing of mixed bacteria. The amorphous a-CoW nanosheets, enriched with oxygen vacancies via acid etching, exhibit a substantially narrowed bandgap (1.22 eV) and markedly enhanced charge separation, producing 22.1-fold higher singlet oxygen under ultrasound (US) irradiation compared with pristine CoW. This ROS burst synergized with CLD achieves potent killing of Staphylococcus aureus (>99.9% at 20 μg mL⁻¹). More critically, ROS disrupt the outer membrane of Gram-negative Escherichia coli, promoting CLD internalization and consequently boosting its lethality by 18.4-fold (from ∼5% to >92.1%). Mechanistic transcriptomics reveals ROS and CLD synergy: ROS disrupt the bacterial membrane via lipid peroxidation, enabling CLD penetration to block protein synthesis and metabolism, while ROS also induce oxidative stress, collectively leading to bacterial death. In a murine wound model co-infected with both pathogens, a-CoW/CLD plus US eradicates >99.9% of mixed bacteria, suppresses systemic inflammation, and significantly accelerates angiogenesis and re-epithelialization. This paradigm establishes a low-dose, broad-spectrum sonodynamic-antibiotic strategy that overcomes antibiotic permeability bottleneck, offering a promising therapeutic avenue against mixed bacterial infections.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthopaedics, The First Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, 541001, PR China.",
    "Department of Electrical Engineering, City University of Hong Kong, Hong Kong SAR, PR China.",
    "State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Licheng Xi"
    },
    {
      "affs": [
        0
      ],
      "name": "Xue Liu"
    },
    {
      "affs": [
        1
      ],
      "name": "Chaojie Yu"
    },
    {
      "affs": [
        2
      ],
      "name": "Tao Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Junyu Liao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanghua Lv"
    },
    {
      "affs": [
        0
      ],
      "name": "Mian Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yajie Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Tingting Hu"
    },
    {
      "affs": [
        2
      ],
      "name": "Ruizheng Liang"
    },
    {
      "affs": [
        0
      ],
      "name": "Chong Shen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/646kqbkvbc53f.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bioactmat.2026.10.010",
  "fulltext_url": "https://europepmc.org/articles/PMC13685460",
  "id": "646kqbkvbc53f",
  "issns": [
    "2452-199X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42910182",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Photodynamic Therapy Research Studies"
    ]
  ],
  "openalex": "W7221653896",
  "pdf_url": "https://europepmc.org/articles/PMC13685460?pdf=render",
  "pmcid": "PMC13685460",
  "pmid": "42910182",
  "published": "2026-10-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/646kqbkvbc53f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42910182",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42910182"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Photodynamic Therapy Research Studies",
      "subfield": "general"
    }
  ],
  "title": "Oxygen-vacancy-rich LDH sonosensitizers potentiate narrow-spectrum antibiotics via membrane-disruption strategy for mixed bacterial wound infections.",
  "title_text": "Oxygen-vacancy-rich LDH sonosensitizers potentiate narrow-spectrum antibiotics via membrane-disruption strategy for mixed bacterial wound infections.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/646kqbkvbc53f",
  "venue": "Bioactive materials",
  "versions": []
}