{
  "abstract": "IntroductionDermatomyositis (DM) is an immune-mediated myopathy characterized by inflammatory and vascular manifestations and variable systemic involvement; however, the interplay among neutrophil activation, complement, and coagulation remains unclear. We investigated whether serum from patients with DM induces tissue factor (TF)-associated neutrophil extracellular traps (NETs) and examined their functional effects in vitro.MethodsSerum from seven patients with active DM and seven matched healthy individuals was analyzed for inflammatory mediators, citrullinated histone H3 (CitH3), thrombin–antithrombin (TAT) complexes, soluble C5b-9, and DNase-I activity. Control neutrophils were exposed to DM serum and, then, NET formation, TF expression and activity, and thrombin generation were assessed. Inhibition experiments targeting complement, TF, and NETs were performed. Co-culture experiments assessed endothelial activation, while skin biopsies were examined by immunofluorescence.ResultsPatients with DM exhibited increased inflammatory mediators, CitH3, TAT complexes, and soluble C5b-9, together with reduced DNase-I activity. DM serum induced TF expression and release of TF-associated NETs by control neutrophils. DNase-I and eculizumab reduced NET formation, while eculizumab additionally decreased TF mRNA expression and NET-associated TF activity. TF-associated NETs promoted adhesion molecule expression by human aortic endothelial cells, an effect attenuated by DNase-I or TF neutralization. TF-positive NET fragments were detected in lesional DM skin but not healthy skin.DiscussionThese exploratory findings support a contribution of C5-dependent complement activation to TF-associated NET formation and show that these NETs can enhance thrombin generation and endothelial activation in vitro. Their relationship to clinical vasculopathy or thrombotic risk remains unknown, providing evidence for potential links between inflammation, thrombosis, and vascular injury in DM.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Maria Ntinopoulou"
    },
    {
      "name": "Lamprini Efstathiou"
    },
    {
      "name": "Aliki I. Venetsanopoulou"
    },
    {
      "name": "Pavlos Simonopoulos"
    },
    {
      "name": "Evangeli Lampri"
    },
    {
      "name": "Nikolaos Koletsos"
    },
    {
      "name": "Nikolaos Zintziovas"
    },
    {
      "name": "Antigoni Sourla"
    },
    {
      "name": "Paraskevi V. Voulgari"
    },
    {
      "name": "Akrivi Chrysanthopoulou"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/64mm0tkbpa1rv.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1926259",
  "fulltext_url": null,
  "id": "64mm0tkbpa1rv",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1926259",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biology/immunology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/64mm0tkbpa1rv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.3389/fimmu.2026.1926259",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1926259"
    },
    {
      "first_seen": "2026-10-30T12:19:19Z",
      "native_id": "10.3389/fimmu.2026.1926259",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1926259/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "feed-journal:biology/immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Tissue factor-associated neutrophil extracellular traps in dermatomyositis: procoagulant activity and endothelial activation in vitro",
  "title_text": "Tissue factor-associated neutrophil extracellular traps in dermatomyositis: procoagulant activity and endothelial activation in vitro",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T00:20:56Z",
  "url": "https://research.draconitic.com/paper/64mm0tkbpa1rv",
  "venue": "Frontiers in Immunology",
  "versions": []
}