{
  "abstract": "N4-acetylcytosine (4acC) is a naturally occurring DNA modification; however, its presence and distribution characteristics in prokaryotes remain unclear. In this study, based on the differential deamination activity of APOBEC3A (A3A) toward cytosine (C) and 4acC, we developed a single-base-resolution sequencing method termed 4acC-A3A-seq (4acC-APOBEC3A-sequencing). Following validation using template strands and optimization of reaction conditions, we applied this method to the genome of Escherichia coli (E. coli) and successfully identified 2271 high-confidence candidate 4acC sites, generating the first single-base-resolution genome-wide map of 4acC in a prokaryote. This study provided a key technological approach and foundational data for further exploration of the biological functions and evolutionary conservation of 4acC at single-base resolution.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Biomedical Polymers-Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yaqian Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xin Xi"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaocheng Weng"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiang Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuhao Du"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/65xegpy7zbbfj.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111570",
  "fulltext_url": null,
  "id": "65xegpy7zbbfj",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1570",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Epigenetics and DNA Methylation"
    ]
  ],
  "openalex": "W7224085573",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/65xegpy7zbbfj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:59Z",
      "native_id": "10.3390/biom16111570",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1570"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Epigenetics and DNA Methylation",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Detection of N4-Acetylcytosine in Escherichia coli Using APOBEC3A-Mediated Deamination Sequencing",
  "title_text": "Detection of N4-Acetylcytosine in Escherichia coli Using APOBEC3A-Mediated Deamination Sequencing",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:59Z",
  "url": "https://research.draconitic.com/paper/65xegpy7zbbfj",
  "venue": "Biomolecules",
  "versions": []
}