{
  "abstract": "Several approaches have been developed to improve the efficiency of CRISPR/Cas9-based genome editing, including the co-inactivation of a gene whose loss confers resistance to a cytotoxic compound, thereby enabling enrichment of successfully edited cells. Here, we show across multiple cell lines that inactivation of HSD17B11, a non-essential member of the Short-chain Dehydrogenase/Reductase (SDR) superfamily, confers strong resistance (29-to 131-fold) to a Phenyl diAlkynylCarbinol compound (PAC) in both human and mouse cells, without affecting cell viability or proliferation. We demonstrate that co-inactivation of HSD17B11 followed by PAC selection can be used to rapidly identify efficient guide RNAs targeting a gene of interest and to readily isolate clones inactivated for one or multiple genes. Altogether, these results establish a simple and efficient experimental strategy for generating knockout cells by using PAC selection to enrich for successfully edited cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Bastien Dumais"
    },
    {
      "name": "Madeleine Bossaert"
    },
    {
      "name": "Patrick Seigneur"
    },
    {
      "name": "Alexandrine Rozié"
    },
    {
      "name": "Sophie Gasmi"
    },
    {
      "name": "Luce Fontanié--Roger"
    },
    {
      "name": "Maëlle Caroff"
    },
    {
      "name": "Valérie Maraval"
    },
    {
      "name": "Vania Bernardes-Génisson"
    },
    {
      "name": "Dennis Gomez"
    },
    {
      "name": "Philippe Frit"
    },
    {
      "name": "Stéphanie Ballereau"
    },
    {
      "name": "Yves Génisson"
    },
    {
      "name": "Sébastien Britton"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/667t2n39t2a4q.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.05.13.724824",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.05.13.724824v2.full",
  "id": "667t2n39t2a4q",
  "issns": [],
  "landing_url": "https://cnrs.hal.science/hal-05772769v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "2.sdv.bbm.bm"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7161142144",
  "pdf_url": "https://cnrs.hal.science/hal-05772769/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-05-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/667t2n39t2a4q.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:45Z",
      "native_id": "hal-05772769",
      "source": "hal",
      "url": "https://cnrs.hal.science/hal-05772769v1"
    },
    {
      "first_seen": "2026-10-30T12:23:26Z",
      "native_id": "10.64898/2026.05.13.724824",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.05.13.724824v2"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "Exploiting HSD17B11-dependent dialkynylcarbinols cytotoxicity for facile CRISPR/Cas9-based gene inactivation",
  "title_text": "Exploiting HSD17B11-dependent dialkynylcarbinols cytotoxicity for facile CRISPR/Cas9-based gene inactivation",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:26Z",
  "url": "https://research.draconitic.com/paper/667t2n39t2a4q",
  "venue": "HAL",
  "versions": []
}