{
  "abstract": "Protein-DNA interactions often result in rotational movements. These movements underlie many genetic processes such as DNA transcription by RNA polymerases (RNAPs). To illuminate these movements, we previously developed origami-rotor-based imaging and tracking (ORBIT), a fluorescence-based method that enables high-speed tracking of DNA rotation. When used to track DNA rotation during transcription by E. coli RNAP, ORBIT enabled the detection of single base-pair steps. However, the duration of ORBIT experiments was limited due to fluorescence photobleaching. To overcome this limitation and extend the total possible observation time, we here introduce dye-cycling ORBIT (dcORBIT), a method that enables observation of protein-DNA interactions for over 10 min at 20 Hz while maintaining single-base-pair precision. dcORBIT thereby opens new possibilities to study the fundamental rotational movements underlying gene expression.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Molecular Biology, University of California, San Diego, 9500 Gillman Drive, La Jolla, CA 92093, USA",
    "Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA",
    "Omenn-Darling Bioengineering Institute, Princeton University, 41 Olden Street, Princeton, NJ 08544, USA"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Amanda L. Wacker"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ryan J. Fantasia"
    },
    {
      "affs": [
        1
      ],
      "name": "Brian Tenner"
    },
    {
      "affs": [
        1
      ],
      "name": "Boyu Liu"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Jerry D. Wu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Nicholas Monell"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pallav Kosuri"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/66c43gc87br2v.bib",
  "cited_by": 0,
  "doi": "10.1016/j.crmeth.2026.101550",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13721745/",
  "id": "66c43gc87br2v",
  "issns": [
    "2667-2375"
  ],
  "landing_url": "https://doi.org/10.1016/j.crmeth.2026.101550",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Report"
    ],
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7202393771",
  "pdf_url": null,
  "pmcid": "PMC13721745",
  "pmid": "42594870",
  "published": "2026-08-13",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/66c43gc87br2v.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:22:23Z",
      "native_id": "PMC13721745",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.crmeth.2026.101550"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Dye-cycling DNA origami rotors for long-term tracking of transcription at base-pair resolution",
  "title_text": "Dye-cycling DNA origami rotors for long-term tracking of transcription at base-pair resolution",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:23Z",
  "url": "https://research.draconitic.com/paper/66c43gc87br2v",
  "venue": "Cell Reports Methods",
  "versions": []
}