{
  "abstract": "Objective: To explore the effects and related mechanisms of a β-blocker combined with a 5-hydroxytryptamine 1A receptor (5-HT1AR) agonist on MI injuries in rats. Methods: TTC/Evans blue, H&E, Masson, and TUNEL staining were used to assess infarction area, pathological damage, fibrosis, and apoptosis in myocardial tissue. The ELISA kits were used to detect IL-1β and IL-18 levels. The immunohistochemical (IHC) staining was used in conjunction with qRT-PCR and Western blot to determine IL-1β, IL-18, NLRP3, pro Caspase-1, cleaved Caspase-1, GSDMD-FL, and GSDMD-N expression levels. Results: In myocardial tissue, the β-blocker combined with the 5-HT1AR agonist effectively reduced infarction size, mitigated pathological injury, alleviated fibrosis, and prevented apoptosis. Meanwhile, the β-blocker combined with the 5-HT1AR agonist downregulated IL-1β and IL-18 levels in serum and myocardial tissue. Notably, although it barely altered the expression of the pyroptosis precursor proteins pro Caspase‑1 and GSDMD‑FL, the combined therapy strongly suppressed the mRNA and protein levels of NLRP3, cleaved Caspase‑1, and GSDMD‑N, and reduced the ratios of pro Caspase‑1/cleaved Caspase‑1 and GSDMD-FL/GSDMD-N in myocardial tissue. Conclusion: β-blocker combined with the 5-HT1AR agonist relieves MI injuries by suppressing the NLRP3/Caspase-1/GSDMD pathway.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Psycho-cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Haiyang Chen"
    },
    {
      "name": "Yanwei Li"
    },
    {
      "name": "Meiyan Liu"
    },
    {
      "name": "Yunpeng Chi"
    },
    {
      "name": "Lijun Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/67479kq36ax1f.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360695",
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  "id": "67479kq36ax1f",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360695",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Inflammasome and immune disorders"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell processes/Cell death/Apoptosis"
    ],
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      "plos",
      "/Biology and life sciences/Developmental biology/Molecular development/Cytokines"
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      "plos",
      "/Biology and life sciences/Immunology/Immune response/Inflammation"
    ],
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      "/Biology and life sciences/Immunology/Immune system/Innate immune system/Cytokines"
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      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Molecular biology assays and analysis techniques/Gene expression and vector techniques/Protein expression"
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    [
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      "/Biology and life sciences/Physiology/Immune physiology/Cytokines"
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    [
      "plos",
      "/Medicine and health sciences/Cardiology/Myocardial infarction"
    ],
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      "/Medicine and health sciences/Clinical medicine/Signs and symptoms/Inflammation"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune response/Inflammation"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune system/Innate immune system/Cytokines"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pharmaceutics/Drug therapy/Receptor antagonist therapy/Beta-adrenergic antagonist therapy"
    ],
    [
      "plos",
      "/Research and analysis methods/Histochemistry and cytochemistry techniques/Immunohistochemistry techniques"
    ],
    [
      "plos",
      "/Research and analysis methods/Immunologic techniques/Immunoassays/Enzyme-linked immunoassays"
    ],
    [
      "plos",
      "/Research and analysis methods/Immunologic techniques/Immunohistochemistry techniques"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Molecular biology assays and analysis techniques/Gene expression and vector techniques/Protein expression"
    ]
  ],
  "openalex": "W7221749075",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0360695&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-14",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/67479kq36ax1f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:23Z",
      "native_id": "10.1371/journal.pone.0360695",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0360695"
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  "subjects": [
    {
      "field": "biology",
      "origin": "plos:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Protection of beta-blocker and 5-hydroxytryptamine 1A receptor agonist on myocardial ischemia injury in rats by regulating the NLRP3/Caspase-1/GSDMD pathway",
  "title_text": "Protection of beta-blocker and 5-hydroxytryptamine 1A receptor agonist on myocardial ischemia injury in rats by regulating the NLRP3/Caspase-1/GSDMD pathway",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:23Z",
  "url": "https://research.draconitic.com/paper/67479kq36ax1f",
  "venue": "PLOS One",
  "versions": []
}