{
  "abstract": "The mechanism of the bacterial diterpene synthase ChtS from Chitinophaga japonensis was investigated. ChtS was found to produce two major diterpenes, 5/11-bicyclic chitino-2,5,9-triene (11) and the previously uncharacterized 6/10-bicyclic chitinoeunicellene (12). Deuterium labeling experiments, site-directed mutagenesis, and isolation of minor products cembrene C (13), cembrene A (14), and chitinol (15) support initial 1,14-cyclization, two sequential 1,2-hydride shifts, prior to bifurcation to 11 or 12, ultimately retaining the E-configured C-2/C-3 alkene.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemistry",
    "University of Florida",
    "BioTools, Inc., 5730 Corporate Way, Suite 220, West Palm Beach, Florida, 33407"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Wenbo Ning"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pin-Shuo Huang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiuting Wei"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Fabrizio G. Cavassa"
    },
    {
      "affs": [
        2
      ],
      "name": "Jordan Nafie"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jeffrey D. Rudolf",
      "orcid": "0000-0003-2718-9651"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/68hawfvg06drz.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15010488/v1",
  "fulltext_url": null,
  "id": "68hawfvg06drz",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010488/v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant biochemistry and biosynthesis"
    ]
  ],
  "openalex": "W7222226450",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15010488/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-14",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/68hawfvg06drz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:24Z",
      "native_id": "10.26434/chemrxiv.15010488",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010488/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant biochemistry and biosynthesis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Mechanistic characterization of a bacterial terpene synthase that bifurcates to produce both 6/10- and 5/11-bicyclic diterpenes",
  "title_text": "Mechanistic characterization of a bacterial terpene synthase that bifurcates to produce both 6/10- and 5/11-bicyclic diterpenes",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:24Z",
  "url": "https://research.draconitic.com/paper/68hawfvg06drz",
  "venue": "ChemRxiv",
  "versions": []
}