{
  "abstract": "Understanding cellular heterogeneity in human skin is crucial for regenerative medicine and tissue engineering. In this study, we applied label-free Raman imaging to visualize molecular features corresponding to the three-dimensional architecture of the epidermis. Spatially resolved Raman spectra, combined with multivariate data analysis, enabled the identification of cell-layer-specific molecular signatures. Based on the region-specific spectra analysis, component C5 was predominantly localized to the basal layer within rete ridges and was characterized by β-sheet-enriched keratin features. This spatially restricted distribution reflects the molecular microenvironment of epidermal stem cell niches, suggesting that C5 may serve as a biomarker for basal stem cell populations associated with skin undulations. These findings provide insight into the molecular basis of epidermal architecture and demonstrate the potential of Raman spectroscopy as a label-free tool for evaluating stem cell localization and differentiation status.",
  "abstract_shown": true,
  "affiliations": [
    "Institute for Advanced Research of Biosystem Dynamics, Waseda Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-Ku, Tokyo 169-8555, Japan.",
    "Research Organization for Nano and Life Innovation, Waseda University, 513 Wasedatsurumaki-Cho, Shinjuku-Ku, Tokyo 162-0041, Japan.",
    "National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.",
    "Division of Skin Regeneration and Aging, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.",
    "International Research Center for Medical Sciences, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.",
    "Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.",
    "Department of Advanced Science Engineering, Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Kaori Sugiyama",
      "orcid": "0000-0002-0049-4499"
    },
    {
      "affs": [
        1
      ],
      "name": "Masahiro Ando"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Mizuho Ishikawa"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Aiko Sada"
    },
    {
      "affs": [
        0,
        1,
        5,
        6
      ],
      "name": "Haruko Takeyama",
      "orcid": "0000-0002-2058-8185"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/68y6pe3qhtst4.bib",
  "cited_by": 0,
  "doi": "10.1021/cbmi.6c00030",
  "fulltext_url": "https://europepmc.org/articles/PMC13715323",
  "id": "68y6pe3qhtst4",
  "issns": [
    "2832-3637"
  ],
  "landing_url": "https://europepmc.org/article/MED/42953695",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Letter"
    ],
    [
      "openalex",
      "1304|Spectroscopy Techniques in Biomedical and Chemical Research"
    ]
  ],
  "openalex": "W7162534253",
  "pdf_url": "https://europepmc.org/articles/PMC13715323?pdf=render",
  "pmcid": "PMC13715323",
  "pmid": "42953695",
  "published": "2026-05-27",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/68y6pe3qhtst4.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:43Z",
      "native_id": "MED:42953695",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953695"
    },
    {
      "first_seen": "2026-11-01T12:22:39Z",
      "native_id": "PMC13715323",
      "source": "pmc",
      "url": "https://doi.org/10.1021/cbmi.6c00030"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Spectroscopy Techniques in Biomedical and Chemical Research",
      "subfield": "biophysics"
    }
  ],
  "title": "Label-Free Raman Imaging Defines Distinct Cell Populations in Human Skin.",
  "title_text": "Label-Free Raman Imaging Defines Distinct Cell Populations in Human Skin.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:39Z",
  "url": "https://research.draconitic.com/paper/68y6pe3qhtst4",
  "venue": "Chemical & biomedical imaging",
  "versions": []
}