{
  "abstract": "Aspirin (ASA), a classical nonsteroidal anti-inflammatory drug, is known to inhibit glycolysis in cancer cells; however, the underlying mechanism remain incompletely understood. Here, we identify lysine salicylation (Ksa) as an ASA-dependent protein modification that suppresses glycolysis and proliferation in colorectal cancer cells. Specifically, ASA causes Ksa on the key glycolytic enzyme aldolase A (ALDOA), markedly reducing its enzymatic activity without altering protein level. Metabolomic analysis revealed that ASA impairs the conversion of fructose-1,6-bisphosphate (F16BP) to glyceraldehyde-3-phosphate (GAP), thereby disrupting glycolytic flux. In a xenograft mouse model, ASA administration not only inhibited tumor growth but also enhanced ALDOA Ksa level and blocked F16BP-to-GAP conversion. Besides, site-directed mutagenesis and genetic code expansion experiments confirmed that ALDOA K13sa is critical for ASA-mediated inhibition of glycolysis and cancer cell proliferation. Together, this study uncovers Ksa as an innovative mechanism by which ASA affects glycolysis and cancer cell proliferation, providing further insights into its potential role in cancer prevention and therapy.",
  "abstract_shown": true,
  "affiliations": [
    "Institute for Advanced Study, Chengdu University, Chengdu 610106, China",
    "National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China",
    "Center for Molecular Oncology, Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China",
    "Shanghai AB SCIEX Analytical Instrument Trading Co., Ltd., Shanghai 200335, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yanqiu Gong"
    },
    {
      "affs": [
        1
      ],
      "name": "Li Gao"
    },
    {
      "affs": [
        1
      ],
      "name": "Aolan Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuqi Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Wenchen Pu"
    },
    {
      "affs": [
        2
      ],
      "name": "Shuangyan Tan"
    },
    {
      "affs": [
        1
      ],
      "name": "Shupan Guo"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanting He"
    },
    {
      "affs": [
        1
      ],
      "name": "Xinyuan Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Qinlu Peng"
    },
    {
      "affs": [
        3
      ],
      "name": "Longlong Hu"
    },
    {
      "affs": [
        2
      ],
      "name": "Yong Peng"
    },
    {
      "affs": [
        1
      ],
      "name": "Haiyan Ren"
    },
    {
      "affs": [
        1
      ],
      "name": "Lunzhi Dai"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/69p8vqm5ggdv8.bib",
  "cited_by": 0,
  "doi": "10.1016/j.apsb.2026.07.002",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729543/?tool=EBI",
  "id": "69p8vqm5ggdv8",
  "issns": [
    "2211-3835",
    "2211-3843"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13729543",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1306|Cancer, Hypoxia, and Metabolism"
    ]
  ],
  "openalex": "W7167505286",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729543/pdf/?tool=EBI",
  "pmcid": "PMC13729543",
  "pmid": "42979438",
  "published": "2026-07-06",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/69p8vqm5ggdv8.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:47Z",
      "native_id": "PMC:PMC13729543",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13729543"
    },
    {
      "first_seen": "2026-11-07T12:22:16Z",
      "native_id": "PMC13729543",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.apsb.2026.07.002"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Cancer, Hypoxia, and Metabolism",
      "subfield": "oncology"
    }
  ],
  "title": "Aspirin induces glycolytic reprogramming via lysine salicylation of ALDOA",
  "title_text": "Aspirin induces glycolytic reprogramming via lysine salicylation of ALDOA",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:22:16Z",
  "url": "https://research.draconitic.com/paper/69p8vqm5ggdv8",
  "venue": "Acta pharmaceutica Sinica. B",
  "versions": []
}