{
  "abstract": "To predict neoadjuvant chemotherapeutic outcomes, breast cancer organoids have been developed. However, low yields from small tissue specimens and the high cost of niche factor-enriched medium remain barriers to clinical translation. Here, breast cancer patient-derived microtumors (PDMs) from core needle biopsies and small tissue specimens were established using a modified culture protocol and an optimized fetal bovine serum (FBS)-based culture medium, achieving a 75% success rate (6 of 8 cases). PDM characteristics mostly agreed with the tissue origins, according to growth evaluation, histopathology, immunohistochemistry, and genetic profiling. As a proof of concept, PDMs were tested with neoadjuvant chemotherapy alongside the patients' treatment. Notably, the PDM results were positively correlated with the patient outcomes and were obtained within a clinically relevant time frame (∼2-3 weeks). Collectively, our protocol and FBS-based medium efficiently generate breast cancer PDMs from core needle biopsies and small tissue specimens, with promising potential to guide neoadjuvant chemotherapy decisions.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Mooktapa Plikomol",
      "orcid": "0009-0006-4727-6663"
    },
    {
      "name": "Suphawat Laohawiriyakamol",
      "orcid": "0000-0002-4055-8784"
    },
    {
      "name": "Piyanun Wangkulangkul",
      "orcid": "0000-0002-0585-3649"
    },
    {
      "name": "Paramee Thongsuksai"
    },
    {
      "name": "Pokphon Khirilak",
      "orcid": "0009-0008-1178-3895"
    },
    {
      "name": "Ratsamaporn Nontikarn"
    },
    {
      "name": "Onpawee Phanthuvet"
    },
    {
      "name": "Karan Ulhaka",
      "orcid": "0000-0002-3555-8210"
    },
    {
      "name": "Pasarat Khongkow",
      "orcid": "0000-0003-3068-2412"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6a1nd8c10bh4m.bib",
  "cited_by": 0,
  "doi": "10.1016/j.isci.2026.117914",
  "fulltext_url": "https://europepmc.org/articles/PMC13694900",
  "id": "6a1nd8c10bh4m",
  "issns": [
    "2589-0042"
  ],
  "landing_url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03297-9",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Cancer"
    ],
    [
      "heading",
      "Cell biology"
    ],
    [
      "openalex",
      "2730|Cancer Cells and Metastasis"
    ]
  ],
  "openalex": "W7222237329",
  "pdf_url": "https://europepmc.org/articles/PMC13694900?pdf=render",
  "pmcid": "PMC13694900",
  "pmid": "42927220",
  "published": "2026-10-15",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/6a1nd8c10bh4m.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:16Z",
      "native_id": "10.1016/j.isci.2026.117914",
      "source": "feed-cell",
      "url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03297-9"
    },
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1016/j.isci.2026.117914",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2589004226032979"
    },
    {
      "first_seen": "2026-10-30T12:21:18Z",
      "native_id": "MED:42927220",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42927220"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "medicine",
      "origin": "heading:Cancer",
      "subfield": "oncology"
    }
  ],
  "title": "Core needle biopsy-derived breast cancer microtumors enable functional drug-response testing for precision oncology",
  "title_text": "Core needle biopsy-derived breast cancer microtumors enable functional drug-response testing for precision oncology",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:18Z",
  "url": "https://research.draconitic.com/paper/6a1nd8c10bh4m",
  "venue": "iScience",
  "versions": []
}