{
  "abstract": "Ribosomal proteins, because of their RNA-binding capacity, may engage various cellular RNAs and fulfill non-ribosomal roles. Previously, we and others described the intergenic regulation mediated by splicing of RPL22 paralogs in Saccharomyces cerevisiae. Here, we prepared a panel of RPL22A/B intronic mutants with respect to their RNAfold-predicted features and analyzed their properties. We tested splicing efficiency and Rpl22-intron binding using an intron-containing reporter and a yeast three-hybrid system, respectively. We found that the splicing of RPL22 introns can be inhibited by stabilizing a predicted stem as part of a particular type of conformation (I structure). Stabilizing the formation of an alternate stem (P structure) permitted splicing. Binding of the Rpl22 protein to the intron led to enhanced splicing inhibition in WT and several of the mutants, which we interpret as stabilization of the I structure. Mutagenesis identified both the main and alternative 5’ss and additional stem loops as part of the regulatory mechanism. The inhibitory conformation of the intron did not prevent recognition of the 5’ss and branch point, but rather stalled splicing at a later stage, before the first catalytic step. We conclude that the structural ensemble of the RPL22 pre-mRNA behaves as an allosteric switch that responds to Rpl22 concentration.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cell Biology, Faculty of Science, Charles University, Praha, Czech Republic"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kateřina Abrhámová"
    },
    {
      "name": "Alexandra Gredová"
    },
    {
      "name": "Karolína Navrátilová"
    },
    {
      "name": "Mohamed Boumaiza"
    },
    {
      "name": "Petr Folk"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6a4er0m1nmasx.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0359197",
  "fulltext_url": null,
  "id": "6a4er0m1nmasx",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0359197",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA processing/RNA splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA structure"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA structure/RNA stem-loop structure"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Spliceosomes"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Protein structure/Protein structure prediction"
    ],
    [
      "plos",
      "/Biology and life sciences/Computational biology/Genome complexity/Introns"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/RNA processing/RNA splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome complexity/Introns"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Macromolecular structure analysis/Protein structure/Protein structure prediction"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Macromolecular structure analysis/RNA structure"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Macromolecular structure analysis/RNA structure/RNA stem-loop structure"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Fungi/Yeast"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Fungi/Yeast/Saccharomyces/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Yeast and fungal models/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Model organisms/Saccharomyces cerevisiae"
    ]
  ],
  "openalex": "W7215101530",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0359197&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/6a4er0m1nmasx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:37Z",
      "native_id": "10.1371/journal.pone.0359197",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0359197"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch",
  "title_text": "Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:37Z",
  "url": "https://research.draconitic.com/paper/6a4er0m1nmasx",
  "venue": "PLOS One",
  "versions": []
}