{
  "abstract": "The widespread use of propofol in pediatric anesthesia raises significant concerns regarding its potential neurodevelopmental toxicity and long-term cognitive impairment in children. To explore the protective role of miR-432-5p against propofol-induced neurotoxicity and clarify its underlying mechanisms mediated by targeting interleukin-2-inducible T-cell kinase (ITK). An in vitro model of propofol-induced damage was constructed using the SH-SY5Y cell line. Cell survival, the percentage of apoptosis, and the secretion of pro-inflammatory factors (TNF-α, IL-1β, and IL-6) were subsequently quantified. Finally, the direct interaction between miR-432-5p and its target, ITK, was verified through dual-luciferase reporter assays. miR-432-5p was markedly downregulated following propofol exposure. In vitro, miR-432-5p overexpression improved cell viability, reduced apoptosis, and suppressed pro-inflammatory cytokine release. ITK was validated as a direct downstream target of miR-432-5p. Furthermore, compensatory overexpression of ITK partially reversed the anti-apoptotic and anti-inflammatory protective phenotypes induced by miR-432-5p. The miR-432-5p/ITK regulatory axis significantly mitigates propofol-induced cellular injury by restraining apoptosis and neuroinflammation. Maintaining miR-432-5p abundance provides a promising theoretical basis and therapeutic target for preventing anesthesia-related cognitive impairment.",
  "abstract_shown": true,
  "affiliations": [
    "Anesthesiology Department, Aoyang Hospital Affiliated to Jiangsu University, Suzhou, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Aijing Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Changke Li",
      "orcid": "0009-0009-2792-416X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6av1q6xzka4t4.bib",
  "cited_by": 0,
  "doi": "10.1002/jbt.71153",
  "fulltext_url": "https://europepmc.org/articles/PMC13724207",
  "id": "6av1q6xzka4t4",
  "issns": [
    "1095-6670",
    "1099-0461"
  ],
  "landing_url": "https://europepmc.org/article/MED/42967583",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2806|Anesthesia and Neurotoxicity Research"
    ]
  ],
  "openalex": "W7225038060",
  "pdf_url": "https://europepmc.org/articles/PMC13724207?pdf=render",
  "pmcid": "PMC13724207",
  "pmid": "42967583",
  "published": "2026-11-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6av1q6xzka4t4.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:53Z",
      "native_id": "MED:42967583",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42967583"
    },
    {
      "first_seen": "2026-11-04T12:22:42Z",
      "native_id": "PMC13724207",
      "source": "pmc",
      "url": "https://doi.org/10.1002/jbt.71153"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2806|Anesthesia and Neurotoxicity Research",
      "subfield": "neuroscience"
    }
  ],
  "title": "Protective Role of miR-432-5p Against Propofol-Induced Neurotoxicity Through Regulation of ITK in SH-SY5Y Cells.",
  "title_text": "Protective Role of miR-432-5p Against Propofol-Induced Neurotoxicity Through Regulation of ITK in SH-SY5Y Cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:53Z",
  "url": "https://research.draconitic.com/paper/6av1q6xzka4t4",
  "venue": "Journal of biochemical and molecular toxicology",
  "versions": []
}