{
  "abstract": "The E3 ubiquitin ligase TRIM23 is involved in diverse cellular processes, however, its function in antiviral defense against adenovirus remains unclear. Here, we identify a novel mechanism by which TRIM23 restricts human adenovirus type 5 (HAdV-C5) replication. TRIM23 expression was upregulated upon HAdV-C5 infection, and functional studies showed that its overexpression inhibited viral replication, while knockdown enhanced it. Mechanistically, TRIM23 interacts with the viral E1A protein and promotes its degradation through a mechanism dependent of its canonical E3 ligase activity. Moreover, TRIM23 recruits the selective autophagy receptor p62 promotes E1A degradation in a E1A ubiquitination-independent manner. Our results unveil a novel host defense pathway-the TRIM23-E1A-p62 axis-that highlights the role of selective autophagy in antiviral immunity. Author summary: Elucidating virus-host interactions is critical for developing therapies against HAdV-C5. This study reveals that TRIM23 plays an antiviral role independent of its ubiquitination function. Depending on its E3 ligase activity, TRIM23 counteracts HAdV-C5 by directing viral E1A to autophagic degradation via p62, thereby circumventing typical ubiquitin-proteasome mediated signaling. These findings expand our understanding of how selective autophagy restricts viral infection and uncover a previously unrecognized layer of host defense centered on the TRIM23-E1A-p62 axis.",
  "abstract_shown": true,
  "affiliations": [
    "Cancer Institute, Cellular Therapeutics School of Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China",
    "Center of Clinical Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China",
    "Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou Medical University, Xuzhou, Jiangsu, China",
    "Department of Pathogen Biology and Immunology, Jiangsu Key Laboratory of Immunity and Metabolism, School of Basic Medical Sciences, Xuzhou Medical University, Xuzhou, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nan Sun"
    },
    {
      "affs": [
        1
      ],
      "name": "Jikai Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Banruo Xia"
    },
    {
      "affs": [
        3
      ],
      "name": "Hang Yin"
    },
    {
      "name": "Jiaqian Xie"
    },
    {
      "name": "Xiaoxiao Liu"
    },
    {
      "name": "Man Li"
    },
    {
      "name": "Lin Fang"
    },
    {
      "name": "Guijuan Ji"
    },
    {
      "name": "Gaolei Ma"
    },
    {
      "name": "Haihan Zhang"
    },
    {
      "name": "Zhenzhen Wang"
    },
    {
      "name": "Dafei Chai"
    },
    {
      "name": "Gang Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6avngqedxtq2b.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.ppat.1014578",
  "fulltext_url": "https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1014578",
  "id": "6avngqedxtq2b",
  "issns": [
    "1553-7374"
  ],
  "landing_url": "https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1014578",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Autophagy in Disease and Therapy"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell processes/Cell death/Autophagic cell death"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Viral pathogens/Adenoviruses"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Virology/Viral replication"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/DNA construction/Plasmid construction"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Transfection"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Viruses/DNA viruses/Adenoviruses"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Viruses/Viral pathogens/Adenoviruses"
    ],
    [
      "plos",
      "/Medicine and health sciences/Nephrology/Renal cancer"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Genitourinary tract tumors/Prostate cancer"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Viral pathogens/Adenoviruses"
    ],
    [
      "plos",
      "/Medicine and health sciences/Urology/Prostate diseases/Prostate cancer"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/DNA construction/Plasmid construction"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Transfection"
    ],
    [
      "plos",
      "/Research and analysis methods/Precipitation techniques/Immunoprecipitation/Co-immunoprecipitation"
    ]
  ],
  "openalex": "W7211950929",
  "pdf_url": "https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1014578&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-08",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/6avngqedxtq2b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "10.1371/journal.ppat.1014578",
      "source": "plos",
      "url": "https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1014578"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "plos:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "TRIM23 prevents adenovirus replication by p62-mediated selective autophagic degradation of viral E1A protein",
  "title_text": "TRIM23 prevents adenovirus replication by p62-mediated selective autophagic degradation of viral E1A protein",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/6avngqedxtq2b",
  "venue": "PLOS Pathogens",
  "versions": []
}