{
  "abstract": "Background\n\nSublingual immunotherapy (SLIT) is safe and one of the most effective etiological treatment for pollinosis. Allergen-specific salivary secretory immunoglobulin A (SIgA) is a useful marker to objectively evaluate treatment efficacy. However, saliva samples usually exhibit high background noise because of non-specific binding in enzyme-linked immunosorbent assays (ELISAs). We therefore aimed to establish an amplified ELISA to measure allergen-specific salivary SIgA levels to assess the efficacy of SLIT for Japanese cedar pollinosis.Methods\n\nBiotinyl-tyramide (BT-Tyr) was used to amplify the signal in a colorimetric ELISA. The allergen of Japanese cedar pollen, Sugi basic protein (SBP), was biotinylated and used as a reporter to obtain sensitive and specific results with low background. To obtain preliminary observations, we applied this ELISA for measuring anti-SBP SIgA levels in saliva samples from SLIT-treated patients with Japanese cedar pollinosis.Results\n\nThe ELISA was highly sensitive and achieved more than 200-fold signal amplification compared with non-amplified conditions. It was also specific for antigens without background noise. The levels of SBP-specific SIgA in saliva increased in patients 9 months after SLIT initiation at the latest.Conclusions\n\nWe developed a sensitive colorimetric ELISA to measure SBP-specific SIgA in saliva using BT-Tyr-based amplification and biotinylated antigen as a reporter. We successfully applied this sensitive ELISA to monitor the levels of SBP-specific SIgA in saliva samples from patients with pollinosis receiving SLIT. Increased salivary SBP-specific SIgA levels were observed in these patients.",
  "abstract_shown": true,
  "affiliations": [
    "Research Center, EPS Holdings, Inc., Bunkyo-ku, Tokyo, Japan.",
    "Research Center, EPS Innovative Medicine (Japan) Co., Ltd., Shinjuku-ku, Tokyo, Japan.",
    "Intelligence and Technology Lab Inc., Kaizu, Gifu, Japan.",
    "Biomedical Institute, NPO Primate Agora, Kaizu, Gifu, Japan.",
    "Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Tetsuro Yamamoto"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Fusako Mitsunaga"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Atsushi Kotani"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kazuki Tajima"
    },
    {
      "affs": [
        4
      ],
      "name": "Muneki Hotomi"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Shin Nakamura"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6c09e7fmyfkyx.bib",
  "cited_by": 0,
  "doi": "10.3389/falgy.2026.1857343",
  "fulltext_url": "https://europepmc.org/articles/PMC13678487",
  "id": "6c09e7fmyfkyx",
  "issns": [
    "2673-6101"
  ],
  "landing_url": "https://europepmc.org/article/MED/42898977",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2723|Allergic Rhinitis and Sensitization"
    ]
  ],
  "openalex": "W7220375514",
  "pdf_url": "https://europepmc.org/articles/PMC13678487?pdf=render",
  "pmcid": "PMC13678487",
  "pmid": "42898977",
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6c09e7fmyfkyx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:37Z",
      "native_id": "MED:42898977",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42898977"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2723|Allergic Rhinitis and Sensitization",
      "subfield": "immunology"
    }
  ],
  "title": "A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT.",
  "title_text": "A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:37Z",
  "url": "https://research.draconitic.com/paper/6c09e7fmyfkyx",
  "venue": "Frontiers in allergy",
  "versions": []
}