{
  "abstract": "Peroxynitrite (ONOO−) is an extremely short‐lived and highly reactive species whose biological effects depend on its local concentration at the site of formation. Consequently, detecting and quantifying ONOO− requires probes that combine high selectivity with well‐defined subcellular localization. The cell membrane is a particularly important yet underexplored interface, as it is the first barrier encountered by extracellularly generated ONOO− and determines how much of this species penetrates into the cell. Such membrane‐proximal ONOO− formation occurs during inflammatory processes and oxidative stress–based therapies, and is especially relevant in cold atmospheric plasma treatment, where complex RONS mixtures interact with the membrane before intracellular effects arise. Here, we report Eu.Chol, a luminescent europium(III) complex that integrates a phenylboronic acid ONOO− reactive motif with a cholesterol anchor to localize the probe within lipid bilayers. We demonstrate that Eu.Chol incorporates efficiently into liposomes and Jurkat cells, enabling selective, membrane‐localized ONOO− detection with minimal response to H2O2. These results establish a modular europium(III)‐based scaffold for targeted ONOO− sensing, enabling potential incorporation of organelle‐specific targeting groups to probe RONS dynamics at defined sub‐cellular sites.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemistry Loughborough University Loughborough UK",
    "Engineering of Molecular Nanosystems, Ecole Polytechnique De Bruxelles Université Libre de Bruxelles (ULB) Brussels Belgium",
    "ZIK Plasmatis Leibniz Institute for Plasma Science and Technology (INP) Greifswald Germany",
    "Department of Hematology and Oncology Greifswald University Medical Center Greifswald Germany",
    "Department of Dermatology, Venerology, and Allergology Rostock University Medical Center Rostock Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jamie Webb"
    },
    {
      "affs": [
        0
      ],
      "name": "Rebecca Tiffany"
    },
    {
      "affs": [
        1
      ],
      "name": "Karolis Norvaisa",
      "orcid": "0000-0003-3219-8739"
    },
    {
      "affs": [
        1
      ],
      "name": "Hennie Valkenier",
      "orcid": "0000-0002-4409-0154"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Lea Miebach"
    },
    {
      "affs": [
        2,
        4
      ],
      "name": "Sander Bekeschus",
      "orcid": "0000-0002-8773-8862"
    },
    {
      "affs": [
        0
      ],
      "name": "Stephen J. Butler",
      "orcid": "0000-0001-8109-3330"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6cn99p1qaj4dk.bib",
  "cited_by": 0,
  "doi": "10.1002/chem.71463",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13716002/",
  "id": "6cn99p1qaj4dk",
  "issns": [
    "0947-6539",
    "1521-3765"
  ],
  "landing_url": "https://doi.org/10.1002/chem.71463",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1607|Molecular Sensors and Ion Detection"
    ]
  ],
  "openalex": "W7171394438",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/chem.71463",
  "pmcid": "PMC13716002",
  "pmid": "42509190",
  "published": "2026-07-27",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6cn99p1qaj4dk.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:23:10Z",
      "native_id": "PMC13716002",
      "source": "pmc",
      "url": "https://doi.org/10.1002/chem.71463"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Molecular Sensors and Ion Detection",
      "subfield": "physical"
    }
  ],
  "title": "Cellular Membrane‐Targeted Europium(III) Probe for Selective and Time‐Resolved Detection of Peroxynitrite",
  "title_text": "Cellular Membrane‐Targeted Europium(III) Probe for Selective and Time‐Resolved Detection of Peroxynitrite",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:23:10Z",
  "url": "https://research.draconitic.com/paper/6cn99p1qaj4dk",
  "venue": "Chemistry (Weinheim an Der Bergstrasse, Germany)",
  "versions": []
}