{
  "abstract": "MicroRNAs (miRNAs) are key regulators of skeletal muscle development and homeostasis. As secretory factors, previous studies have identified and validated a space flight-associated circulating microRNA (miRNA) signature in response to space flight. However, the muscle-derived miRNAs are not clear. Here, we comprehensively analyzed the variations of miRNA during mouse myoblast myogenesis in response to space flight, including expression and secretion. We validated some miRNA expression by qRT-PCR analysis in the limb muscle of a disuse-induced atrophy mouse model, mimicking the unloading following microgravity. Functional assays using miRNA mimics further supported the regulatory roles of some miRNAs in target gene expression, as well as in myoblast proliferation and differentiation. These results suggest that deregulation of these miRNAs may play a functional role in muscle atrophy, and they could be candidate biomarkers of muscle atrophy-associated muscular disease. Importantly, our analysis also suggests that spaceflight may regulate miRNA secretion and retention by modulating RNA-binding proteins (RBPs). Secreted miRNAs originating from skeletal muscle cells have been implicated in various diseases affecting the nervous and skeletal systems. Collectively, these findings highlight the potential role of skeletal muscle-derived miRNAs in systemic adaptation to spaceflight.",
  "abstract_shown": true,
  "affiliations": [
    "Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China",
    "Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai, China",
    "Shanghai Institute of Technical Physics of the Chinese Academy of Sciences, Shanghai, China",
    "Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Wenqing Kong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Mei Ma"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhuoyang Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Linjun Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Ruisen Ma"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Siyi Shen"
    },
    {
      "affs": [
        2
      ],
      "name": "Xiaojun Yan"
    },
    {
      "affs": [
        2
      ],
      "name": "Ping Fan"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhoumin Niu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jingjing Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Tianyuan Qiu"
    },
    {
      "affs": [
        0
      ],
      "name": "Nuo Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuting Wu"
    },
    {
      "affs": [
        2
      ],
      "name": "Tao Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yuying Li",
      "orcid": "0009-0002-3048-4395"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Hao Ying"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6d7cn5b6presj.bib",
  "cited_by": 0,
  "doi": "10.1038/s41526-026-00632-x",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13728116/",
  "id": "6d7cn5b6presj",
  "issns": [
    "2373-8065"
  ],
  "landing_url": "https://doi.org/10.1038/s41526-026-00632-x",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "lcc",
      "QP1-981"
    ],
    [
      "lcc",
      "TP248.13-248.65"
    ],
    [
      "openalex",
      "2737|Spaceflight effects on biology"
    ]
  ],
  "openalex": "W7167921178",
  "pdf_url": "https://www.nature.com/articles/s41526-026-00632-x_reference.pdf",
  "pmcid": "PMC13728116",
  "pmid": "42431907",
  "published": "2026-07-01",
  "publisher": "Nature Publishing Group",
  "ris": "https://research.draconitic.com/paper/6d7cn5b6presj.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T12:23:51Z",
      "native_id": "PMC13728116",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41526-026-00632-x"
    },
    {
      "first_seen": "2026-11-08T14:20:59Z",
      "native_id": "038a6f9f5b554712bb59940a595f427a",
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      "url": "https://doi.org/10.1038/s41526-026-00632-x"
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Spaceflight effects on biology",
      "subfield": "physiology"
    }
  ],
  "title": "Comprehensive analysis of miRNA expression, secretion profile, and functional network during myogenesis in response to spaceflight",
  "title_text": "Comprehensive analysis of miRNA expression, secretion profile, and functional network during myogenesis in response to spaceflight",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T14:20:59Z",
  "url": "https://research.draconitic.com/paper/6d7cn5b6presj",
  "venue": "NPJ Microgravity",
  "versions": []
}