{
  "abstract": "K-12 strains MG1655 and NCM3722. The strains are genetically similar but differ in surface area to volume (SA:V) ratios by up to 30%, maximum growth rates on glucose media by 40%, and overflow-inducing growth rates by 80%. The predictions were tested against experimental evidence including phenomics data, membrane proteomics data, and MG1655 SA:V mutant growth rates. The predictions were remarkably consistent with experimental data and provided a membrane-centric explanation for maximum growth rate, maintenance energy generation, respiration chain efficiency (P/O number), and optimal biomass yield of a strain. These analyses did not consider cytosolic macromolecular crowding, highlighting the distinct properties of the presented theory and gaps in current cell biology literature. Cell geometry and membrane protein crowding are significant biophysical constraints and consideration of both provide a more complete theoretical framework for improved understanding and control of cell biology.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemical and Biological Engineering, Center for Biofilm Engineering Montana State University Bozeman MT USA",
    "Department of Mathematical Sciences Montana State University Bozeman MT USA",
    "Department of Chemical Engineering and Applied Chemistry University of Toronto Canada",
    "Department of Ecology, Evolution, and Behavior University of Minnesota St. Paul MN USA",
    "Department of Biological and Environmental Sciences Carroll College Helena MT USA"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ross P. Carlson",
      "orcid": "0000-0002-2464-7111"
    },
    {
      "affs": [
        1
      ],
      "name": "Tomáš Gedeon",
      "orcid": "0000-0001-5555-6741"
    },
    {
      "affs": [
        2
      ],
      "name": "Mauricio Garcia Benitez",
      "orcid": "0000-0001-8492-8655"
    },
    {
      "affs": [
        0
      ],
      "name": "Campbell Putnam"
    },
    {
      "affs": [
        3
      ],
      "name": "William R. Harcombe",
      "orcid": "0000-0001-8445-2052"
    },
    {
      "affs": [
        2
      ],
      "name": "Radhakrishnan Mahadevan",
      "orcid": "0000-0002-1270-9063"
    },
    {
      "affs": [
        4
      ],
      "name": "Ashley E. Beck",
      "orcid": "0000-0003-1789-3864"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6d9bfawj50t7z.bib",
  "cited_by": 13,
  "doi": "10.1002/1873-3468.70380",
  "fulltext_url": null,
  "id": "6d9bfawj50t7z",
  "issns": [
    "0014-5793",
    "1873-3468"
  ],
  "landing_url": "https://doi.org/10.1002/1873-3468.70380",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7165751446",
  "pdf_url": null,
  "pmcid": "PMC11370460",
  "pmid": "39229203",
  "published": "2026-06-24",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/6d9bfawj50t7z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W7165751446",
      "source": "openalex",
      "url": "https://doi.org/10.1002/1873-3468.70380"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy",
  "title_text": "Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:41Z",
  "url": "https://research.draconitic.com/paper/6d9bfawj50t7z",
  "venue": "FEBS Letters",
  "versions": []
}