{
  "abstract": "Metronidazole is an antiprotozoal antibiotic drug holding promise for tumor microenvironment analysis in hyperpolarized MRI due to its hypoxia-sensitive metabolism. Previous studies have explored the 15N hyperpolarization of metronidazole using dissolution dynamic nuclear polarization (d-DNP) and signal amplification by reversible exchange (SABRE). Here, we demonstrate that by using spin-lock-induced crossing (SLIC), 15N polarization levels up to 10-11% are achieved for 30 mM [15N3]metronidazole, improving polarization levels by a factor of 1.4 ± 0.1 compared to polarization levels achieved in conventional SABRE experiments under otherwise the same conditions. This polarization was harnessed to probe the purification of methanolic solutions of hyperpolarized [15N3]metronidazole, reducing the methanol concentration by 10-fold in the obtained aqueous solutions of 15N-hyperpolarized metronidazole, retaining up to 1/3 of initial polarization. This pilot study marks a substantial step toward the biomedical application of [15N3]metronidazole and is promising for the 15N-hyperpolarization of other substrates via SLIC and other SABRE strategies.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Medical Physics, Department of Diagnostic and Interventional Radiology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg79106, Germany.",
    "German Cancer Consortium (DKTK), Partner Site Freiburg, a Partnership between DKFZ and University Medical Center Freiburg, Im Neuenheimer Feld 280, Heidelberg69120, Germany.",
    "Section Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel24118, Germany.",
    "Department of Chemistry, Integrative Biosciences (Ibio), Karmanos Cancer Institute (KCI), Wayne State University, Detroit, Michigan48202, United States.",
    "NVision Imaging Technologies GmbH, Ulm89081, Germany."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ivan A Trofimov",
      "orcid": "0000-0002-3773-7490"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Henri de Maissin"
    },
    {
      "affs": [
        0
      ],
      "name": "Obaid Mohiuddin"
    },
    {
      "affs": [
        2
      ],
      "name": "Josh P Peters",
      "orcid": "0000-0003-1019-4067"
    },
    {
      "affs": [
        0
      ],
      "name": "Stefan Sell"
    },
    {
      "affs": [
        3
      ],
      "name": "Anna Samoilenko"
    },
    {
      "affs": [
        4
      ],
      "name": "Sebastian Lucas"
    },
    {
      "affs": [
        0
      ],
      "name": "Jochen Leupold"
    },
    {
      "affs": [
        0
      ],
      "name": "Maxim Zaitsev"
    },
    {
      "affs": [
        4
      ],
      "name": "Stephan Knecht",
      "orcid": "0000-0002-8487-4324"
    },
    {
      "affs": [
        2
      ],
      "name": "Andrey N Pravdivtsev"
    },
    {
      "affs": [
        2
      ],
      "name": "Jan-Bernd Hövener",
      "orcid": "0000-0001-7255-7252"
    },
    {
      "affs": [
        3
      ],
      "name": "Eduard Y Chekmenev"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Andreas B Schmidt",
      "orcid": "0000-0001-8944-7463"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6ewjcq87akeg1.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.analchem.6c01373",
  "fulltext_url": "https://europepmc.org/articles/PMC13647987",
  "id": "6ewjcq87akeg1",
  "issns": [
    "0003-2700",
    "1520-6882"
  ],
  "landing_url": "https://europepmc.org/article/MED/42915672",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Advanced NMR Techniques and Applications"
    ]
  ],
  "openalex": "W7207830566",
  "pdf_url": "https://europepmc.org/articles/PMC13647987?pdf=render",
  "pmcid": "PMC13647987",
  "pmid": "42915672",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6ewjcq87akeg1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42915672",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42915672"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced NMR Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Aqueous-Phase Delivery of SLIC-SABRE Hyperpolarized [15N3]Metronidazole.",
  "title_text": "Aqueous-Phase Delivery of SLIC-SABRE Hyperpolarized [15N3]Metronidazole.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/6ewjcq87akeg1",
  "venue": "Analytical chemistry",
  "versions": []
}