{
  "abstract": "Folliculogenesis is essential for fish reproduction, but the role of ERK signaling during early primary follicle development in teleosts remains unclear. Here, we reanalyzed and integrated previously published transcriptomic and DNA methylation datasets from four primary follicle subtypes in zebrafish to identify MAPK-related candidate genes. No new sequencing was performed. Additional qRT-PCR and immunohistochemical analyses examined gene expression and the localization of total ERK and JNK proteins, respectively. Zebrafish were also exposed to the ERK inhibitor SCH772984 from 25 to 60 days post-fertilization to assess changes in ovarian development. The reanalysis identified MAPK-related genes with stage-associated differences in expression and DNA methylation. SCH772984 exposure was associated with reduced ovarian ERK phosphorylation and a lower proportion of advanced follicles. These results support a role for ERK signaling in the progression of early ovarian follicles in zebrafish. These observations derive from a single ERK inhibitor at nominal exposure; complementary assays could strengthen the evidence for target specificity.",
  "abstract_shown": true,
  "affiliations": [
    "College of Life Sciences, Hunan Normal University, Changsha 410081, China.",
    "Henan Academy of Fishery Sciences, Zhengzhou 450044, China.",
    "Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Hunan Academy of Agricultural Sciences, Changsha 410153, China.",
    "Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), Huzhou 313001, China.",
    "Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Guangjing Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jing Huang"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xiudan Yuan"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Yunpeng Fan",
      "orcid": "0000-0003-2374-3627"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Fangyuan Peng"
    },
    {
      "affs": [
        0
      ],
      "name": "Wen Fu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jinhui Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenbin Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Liangyue Peng",
      "orcid": "0000-0003-2128-1801"
    },
    {
      "affs": [
        0
      ],
      "name": "Yamei Xiao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6gnp3h4gxb53s.bib",
  "cited_by": 0,
  "doi": "10.3390/ani16193078",
  "fulltext_url": "https://europepmc.org/articles/PMC13663377",
  "id": "6gnp3h4gxb53s",
  "issns": [
    "2076-2615"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883048",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1314|Reproductive biology and impacts on aquatic species"
    ]
  ],
  "openalex": "W7214958891",
  "pdf_url": "https://europepmc.org/articles/PMC13663377?pdf=render",
  "pmcid": "PMC13663377",
  "pmid": "42883048",
  "published": "2026-09-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6gnp3h4gxb53s.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883048",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883048"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1314|Reproductive biology and impacts on aquatic species",
      "subfield": "physiology"
    }
  ],
  "title": "MAPK Signaling Contributes to Primary Follicle Development in Zebrafish (Danio rerio).",
  "title_text": "MAPK Signaling Contributes to Primary Follicle Development in Zebrafish (Danio rerio).",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/6gnp3h4gxb53s",
  "venue": "Animals : an open access journal from MDPI",
  "versions": []
}