{
  "abstract": "Formalin-fixed paraffin-embedded (FFPE) tissues are archived resources for biomedical research but challenging for single-cell transcriptomic analysis. Here, we present a protocol for isolating high-quality nuclei from FFPE pancreatic cancer sections. We describe steps for deparaffinization, enzymatic digestion, nuclei purification, and library construction using the 10× Genomics Fixed RNA Profiling platform. This protocol enables transcriptomic profiling of archived clinical specimens at single-nucleus resolution. For complete details on the use and execution of this protocol, please refer to Chen et al.¹.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. Electronic address: vivian90@hku.hk.",
    "Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China.",
    "Biomedical Pioneering Innovation Center (BIOPIC), Academy for Advanced Interdisciplinary Studies, and School of Life Sciences, Peking University, Beijing 100871, China.",
    "Institute of Innovative Drug, China Pharmaceutical University, Nanjing 211134, China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China. Electronic address: chenmm_szbl@outlook.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Huiheng Ning"
    },
    {
      "affs": [
        1
      ],
      "name": "Wei Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Kang Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Qinhang Gao"
    },
    {
      "affs": [
        1
      ],
      "name": "Jieying Pan"
    },
    {
      "affs": [
        3
      ],
      "name": "Min-Min Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6h87b792z2jrb.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104880",
  "fulltext_url": "https://europepmc.org/articles/PMC13676707",
  "id": "6h87b792z2jrb",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42848517",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Pancreatic and Hepatic Oncology Research"
    ]
  ],
  "openalex": "W7220929076",
  "pdf_url": "https://europepmc.org/articles/PMC13676707?pdf=render",
  "pmcid": "PMC13676707",
  "pmid": "42848517",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6h87b792z2jrb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42848517",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42848517"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Pancreatic and Hepatic Oncology Research",
      "subfield": "oncology"
    }
  ],
  "title": "Protocol for isolating high-quality nuclei from FFPE pancreatic cancer sections and constructing sequencing libraries.",
  "title_text": "Protocol for isolating high-quality nuclei from FFPE pancreatic cancer sections and constructing sequencing libraries.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/6h87b792z2jrb",
  "venue": "STAR protocols",
  "versions": []
}