{
  "abstract": "Background: Rvi6, introgressed from Malus floribunda 821, is the most widely deployed apple scab resistance gene, but Venturia inaequalis races overcoming it are widespread. Transcriptomic differences between Rvi6-effective and Rvi6-breaking interactions in one host genotype remain unresolved. Aims: To compare these interactions in cv. Ametyst and nominate genes for subsequent validation. Methods: Own-rooted Ametyst plants were mock-inoculated or inoculated with an Rvi6-avirulent isolate (Rin; incompatible) or an Rvi6-virulent isolate (Rola; compatible) and harvested at 4 days post-inoculation (21 mRNA libraries). Expression was quantified using GDDH13 reference-based and de novo Trinity strategies, followed by DESeq2, WGCNA and GSEA. Results: Rin vs Rola yielded 1,339 candidate genes (more than two-fold change, FDR < 0.01) in the reference-based analysis. Curation of Rin-induced defense candidates retained 151 genes, 92 of which concentrated in a single co-expression module. Thirty-one also passed the Rin-vs-Rola thresholded test and were prioritized for genetic validation. Conclusions: The incompatible interaction was associated with a coherent defense program and the compatible interaction with a distinct transcriptional state. With one isolate per class, isolate identity and Rvi6 virulence cannot be separated.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Experimental Botany, Czech Academy of Sciences"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "S. Kertbundit"
    },
    {
      "name": "D. Tyc"
    },
    {
      "name": "R. Cerny"
    },
    {
      "name": "H. Storchova"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Juricek"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6kdkbah51wfq1.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.15.751701",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.15.751701v1.full",
  "id": "6kdkbah51wfq1",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.15.751701v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1110|Plant Disease Resistance and Genetics"
    ]
  ],
  "openalex": "W7213498324",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.15.751701v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6kdkbah51wfq1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:55Z",
      "native_id": "10.64898/2026.09.15.751701",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.15.751701v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Transcriptome of apple cv. Ametyst in Rvi6-effective and Rvi6-breaking Venturia inaequalis interactions identifies defense candidates",
  "title_text": "Transcriptome of apple cv. Ametyst in Rvi6-effective and Rvi6-breaking Venturia inaequalis interactions identifies defense candidates",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:55Z",
  "url": "https://research.draconitic.com/paper/6kdkbah51wfq1",
  "venue": "bioRxiv",
  "versions": []
}