{
  "abstract": "KRAS G12C is an actionable alteration in non-small cell lung cancer, making accurate molecular identification essential for treatment selection. A 61-year-old man with metastatic lung adenocarcinoma showed a KRAS G12C-positive reaction on the AmoyDx Pan Lung Cancer PCR Panel (AmoyDx PLC panel). After progression on first-line chemoimmunotherapy, sotorasib was administered, but progressive disease was observed at the first radiologic assessment. Subsequent NGS-based comprehensive genomic profiling of the same pretreatment FFPE block identified KRAS G12Y (c.34_35delinsTA; variant allele frequency, 25.8%), with no sequence reads supporting KRAS G12C. This prompted suspicion of a false-positive PCR result. Repeat AmoyDx PLC panel testing reproduced the G12C-positive reaction and testing of a KRAS G12Y plasmid likewise yielded a G12C-positive reaction without additional KRAS-associated signals, confirming cross-reactivity. KRAS G12Y can therefore mimic genuine KRAS G12C in mutation-specific PCR testing and may lead to inappropriate genotype-directed therapy.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Respiratory Medicine Graduate School of Medicine, The University of Tokyo Tokyo Japan.",
    "Next-Generation Precision Medicine Development Laboratory Graduate School of Medicine, The University of Tokyo Tokyo Japan."
  ],
  "article_type": "case study",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Tsugumi Iwasaki"
    },
    {
      "affs": [
        0
      ],
      "name": "Hiroaki Ikushima",
      "orcid": "0000-0002-7053-2841"
    },
    {
      "affs": [
        0
      ],
      "name": "Mikako Nakamura"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu Mikami",
      "orcid": "0000-0003-3262-2958"
    },
    {
      "affs": [
        1
      ],
      "name": "Kousuke Watanabe"
    },
    {
      "affs": [
        0
      ],
      "name": "Masanori Kawakami",
      "orcid": "0000-0003-0207-7434"
    },
    {
      "affs": [
        0
      ],
      "name": "Akihisa Mitani"
    },
    {
      "affs": [
        0
      ],
      "name": "Hidenori Kage",
      "orcid": "0000-0003-3658-4442"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6khny9n1551cf.bib",
  "cited_by": 0,
  "doi": "10.1002/rcr2.70805",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13727365/?tool=EBI",
  "id": "6khny9n1551cf",
  "issns": [
    "2051-3380"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13727365",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Case Report"
    ],
    [
      "openalex",
      "2740|Lung Cancer Treatments and Mutations"
    ]
  ],
  "openalex": "W7225107218",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13727365/pdf/?tool=EBI",
  "pmcid": "PMC13727365",
  "pmid": "42974405",
  "published": "2026-11-01",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/6khny9n1551cf.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:51Z",
      "native_id": "PMC:PMC13727365",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13727365"
    },
    {
      "first_seen": "2026-11-04T15:20:47Z",
      "native_id": "MED:42974405",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974405"
    },
    {
      "first_seen": "2026-11-05T12:23:27Z",
      "native_id": "PMC13727365",
      "source": "pmc",
      "url": "https://doi.org/10.1002/rcr2.70805"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Lung Cancer Treatments and Mutations",
      "subfield": "general"
    }
  ],
  "title": "KRAS G12Y Causing a False‐Positive KRAS G12C Result With an Indistinguishable PCR Pattern in Lung Adenocarcinoma",
  "title_text": "KRAS G12Y Causing a False‐Positive KRAS G12C Result With an Indistinguishable PCR Pattern in Lung Adenocarcinoma",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:23:03Z",
  "url": "https://research.draconitic.com/paper/6khny9n1551cf",
  "venue": "Respirology case reports",
  "versions": []
}