{
  "abstract": "Objective To investigate the effect of DJK-5 on the bactericidal efficacy and cariogenic virulence factors of Streptococcus mutans (S. mutans) biofilms. Methods S. mutans suspensions were treated with DJK-5 at concentrations of 0, 5, 10, 20, and 40 μg/mL, and bacterial growth was observed. S. mutans biofilms were treated with sterile deionized water (Control group), 2% chlorhexidine (CHX group) and DJK-5 at concentrations of 10, 20, 40 μg/mL. The structural changes in biofilm were observed using laser confocal microscopy. The production of water-insoluble extracellular polysaccharide (IEPS) and pH values were quantified using the anthrone-sulfuric acid method and a pH meter, respectively. Additionally, the effects of 10 μg/mL DJK-5 on the expression of cariogenic virulence genes in S. mutans biofilms were detected by RT-qPCR. Results DJK-5 at concentrations ≥10 μg/mL exhibited an inhibitory effect on the growth of planktonic S. mutans. The higher the concentration of DJK-5, the more pronounced the inhibitory effect. The volume ratios of dead bacteria in the DJK-5 groups were significantly higher than those in the control and chlorhexidine group. The disruption of spatial structure became more severe with increasing DJK-5 concentrations. The pH values in DJK-5-treated biofilms were significantly higher than those in the control group, and the pH levels in the 20 and 40 μg/mL DJK-5 groups were significantly higher than those in the NaF group. The production of IEPS in DJK-5-treated biofilms was significantly lower than that in the control group. The 10 μg/mL DJK-5 group produced significantly more IEPS than the 40 μg/mL DJK-5 group and the NaF group. DJK-5 promoted the expression of gtfC and gbpB genes, while suppressed the expression of ldh, comDE, and relA genes. Conclusion DJK-5 significantly inhibits acid production and IEPS synthesis in biofilms by targeting and modulating the cariogenic virulence factors of S. mutans, thereby demonstrating highly efficient bactericidal activity against biofilms.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthodontics, The Affiliated Stomatological Hospital of Jiujiang University, Jiujiang 332000, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 1,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "HONG Feng, CHENG Junxiang, DUAN Qinyan, PAN Le, HU Yajun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6m9q689e9f4dy.bib",
  "cited_by": 0,
  "doi": "10.13591/j.cnki.kqyx.2026.10.006",
  "fulltext_url": null,
  "id": "6m9q689e9f4dy",
  "issns": [
    "1003-9872"
  ],
  "landing_url": "https://doi.org/10.13591/j.cnki.kqyx.2026.10.006",
  "language": "zh",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RK1-715"
    ],
    [
      "lcc",
      "RZ201-999"
    ],
    [
      "openalex",
      "3506|Oral microbiology and periodontitis research"
    ]
  ],
  "openalex": "W7224969809",
  "pdf_url": "https://www.stomatology.cn/fileup/1003-9872/PDF/1792136912715-873263540.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": "Editorial Office of Stomatology",
  "ris": "https://research.draconitic.com/paper/6m9q689e9f4dy.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T14:22:05Z",
      "native_id": "0bf71e0b1d9d4204ab83ae947d4a45cf",
      "source": "doaj",
      "url": "https://doi.org/10.13591/j.cnki.kqyx.2026.10.006"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:RK1-715",
      "subfield": "general"
    }
  ],
  "title": "Effects of antimicrobial peptide DJK-5 on cariogenic virulence factors of Streptococcus mutans biofilm",
  "title_text": "Effects of antimicrobial peptide DJK-5 on cariogenic virulence factors of Streptococcus mutans biofilm",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T14:22:05Z",
  "url": "https://research.draconitic.com/paper/6m9q689e9f4dy",
  "venue": "Kouqiang yixue",
  "versions": []
}