{
  "abstract": "TIGR--Tas systems are a recently described class of RNA-guided DNA-targeting systems whose activity has been demonstrated in bacterial and human cells, but not in photosynthetic eukaryotes. Here we tested TIGR--Tas in the green alga Chlamydomonas reinhardtii. Recombinant TaTas and ParTas assembled with in vitro-transcribed tigRNAs cleaved target DNA in a concentration-dependent manner, and electroporated TaTas protein was detected in C. reinhardtii cells. Targeted deep sequencing of the endogenous MAA7 locus recovered indel-containing reads in ParTas-treated cells that were absent from wild-type controls, including a 1-bp deletion located at the position previously reported for ParTas-associated cleavage. Co-delivery of a double-stranded non-homologous oligonucleotide (dsNHO) with a 24-bp duplex and 8-nt 3' overhangs, designed to match the staggered ends generated by TasR-family nucleases, increased the recovery of indel-containing reads for both proteins. The frequency of these events was very low, and the present data do not establish TIGR--Tas as an efficient genome-editing system in this host. They nonetheless provide initial evidence that TIGR--Tas can act on the nuclear genome of a photosynthetic eukaryote, and indicate that dsNHO-assisted recovery of sequence alterations is not restricted to conventional CRISPR--Cas nucleases.",
  "abstract_shown": true,
  "affiliations": [
    "Duke Kunshan University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "L. Jaeyeon"
    },
    {
      "name": "K. Kyeongjun"
    },
    {
      "name": "R. Hyeonmin"
    },
    {
      "name": "Y. Hyeonsik"
    },
    {
      "name": "D. Kerstin"
    },
    {
      "name": "P. Mid-Eum"
    },
    {
      "name": "Y. Joong-Tak"
    },
    {
      "name": "K. Bon-Kyoung"
    },
    {
      "name": "Y. Ji-Hye"
    },
    {
      "name": "L. Ho-Seok"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Eun Yu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6mckv57348yg1.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.12.750307",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.12.750307v1.full",
  "id": "6mckv57348yg1",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.12.750307v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7213224497",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.12.750307v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-12",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6mckv57348yg1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:38Z",
      "native_id": "10.64898/2026.09.12.750307",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.12.750307v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "First evidence for TIGR-Tas activity in a photosynthetic eukaryote",
  "title_text": "First evidence for TIGR-Tas activity in a photosynthetic eukaryote",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:38Z",
  "url": "https://research.draconitic.com/paper/6mckv57348yg1",
  "venue": "bioRxiv",
  "versions": []
}