{
  "abstract": "Fatigue is characterized as a feeling of exhaustion or lack of energy, is a common symptom of multiple chronic illnesses and interferes with daily activities and quality of life. There is a limited availability of animal models to examine potential underlying mechanisms, ultimately limiting development of potential therapeutic strategies. The primary purpose of this study was to develop a mouse model of persistent fatigue. A secondary purpose was to characterize potential measures of “fatigue-like” behaviors. An exploratory goal was to examine for immune and metabolic changes in the model. We examined voluntary wheel running and open field as measures of physical fatigue, and muscle and paw sensitivity as measures of pain. We also examined immune cell phenotype and plasma metabolite profiles after development of persistent fatigue. Acute stress paired with LPS or saline reduced wheel running compared to LPS alone or stress alone. Animals that received acute stress and LPS, showed a decreased ratio of T-helper to T-cytotoxic cells and reduced fatty acid metabolites 10 days after induction; there were no changes in the other groups. Thus, we characterized two unique methods, each requiring multiple stressors, to induce long-lasting fatigue-like behaviors that were associated with different mechanistic changes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Physical Therapy & Rehabilitation Sciences, University of Iowa",
    "Department of Anesthesiology, Duke University School of Medicine",
    "Fraternal Order of Eagles Diabetes Research Center, Carver College of Medicine, University of Iowa",
    "Flow Cytometry Core, Carver College of Medicine, University of Iowa",
    "Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Adam J. Janowski"
    },
    {
      "affs": [
        0
      ],
      "name": "Kazuhiro Hayashi"
    },
    {
      "affs": [
        0
      ],
      "name": "Ashley N. Plumb"
    },
    {
      "affs": [
        0
      ],
      "name": "Joseph B. Lesnak"
    },
    {
      "affs": [
        0
      ],
      "name": "Angela F. Smith"
    },
    {
      "affs": [
        0
      ],
      "name": "Lynn Rasmussen"
    },
    {
      "affs": [
        0
      ],
      "name": "Elizabeth Gross"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrew A. Post"
    },
    {
      "affs": [
        0
      ],
      "name": "Giovanni Berardi"
    },
    {
      "affs": [
        1
      ],
      "name": "Marguerita E. Klein"
    },
    {
      "affs": [
        2
      ],
      "name": "Reid Brown"
    },
    {
      "affs": [
        3
      ],
      "name": "Heath Vignes"
    },
    {
      "affs": [
        4
      ],
      "name": "Eric B. Taylor"
    },
    {
      "affs": [
        1
      ],
      "name": "Andrea G. Nackley"
    },
    {
      "affs": [
        0
      ],
      "name": "Kathleen A. Sluka"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6mewy92z338rs.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-62864-x",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-62864-x",
  "id": "6mewy92z338rs",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-62864-x",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2738|Fibromyalgia and Chronic Fatigue Syndrome Research"
    ]
  ],
  "openalex": "W7169844124",
  "pdf_url": "https://www.nature.com/articles/s41598-026-62864-x_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/6mewy92z338rs.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:42Z",
      "native_id": "3595a95c43b1408dabe4784aa43e4315",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-62864-x"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2738|Fibromyalgia and Chronic Fatigue Syndrome Research",
      "subfield": "psychiatry"
    }
  ],
  "title": "Behavioral and molecular characterization of fatigue-like manifestations triggered by acute restraint stress and LPS or saline",
  "title_text": "Behavioral and molecular characterization of fatigue-like manifestations triggered by acute restraint stress and LPS or saline",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:42Z",
  "url": "https://research.draconitic.com/paper/6mewy92z338rs",
  "venue": "Scientific Reports",
  "versions": []
}