{
  "abstract": "Objective: The insulin receptor (IR) and the insulin‐like growth factor I receptor (IGF‐IR) regulate adipose tissue (AT) differentiation. This study investigated the expression of IGF‐IR, IR, and the IR isoforms (IRa and IRb) in human subcutaneous (S‐ASC) and visceral (V‐ASC) adipose‐derived stem cells.\n\nMethods: S‐AT and V‐AT biopsies were obtained from human donors and ASC were differentiated into adipocytes. mRNA levels of all receptors were quantified by qPCR at baseline and after differentiation. IR protein expression and adipogenic efficiency were assessed by flow cytometry.\n\nResults: In undifferentiated ASC, IGF‐IR transcripts exceeded IR and were higher in S‐ASC than V‐ASC. Upon differentiation, IGF‐IR decreased in S‐ASC but remained unchanged in V‐ASC, whereas IR increased in both depots. S‐ASC displayed ~twofold higher IRa expression and greater insulin‐induced Akt activation. During adipogenesis, IR expression rose in both depots, with IRb exceeding IRa particularly in V‐ASC (+50%). In whole AT, IR exceeded IGF‐IR, and no depot‐specific differences were observed between IR isoforms. Adipogenic conversion was higher in S‐ASC (60%) than V‐ASC (20%). Only ~77% of S‐ASC‐ and ~61% of V‐ASC‐derived adipocytes expressed surface IR.\n\nConclusions: Depot‐specific dynamics of IR isoforms may influence adipogenic capacity and insulin responsiveness and suggest partially receptor‐independent pathways of adipocyte differentiation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Precision and Regenerative Medicine and Ionian Area‐Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases University of Bari Aldo Moro Bari Italy",
    "Section of Endocrinology, Department of Medicine and Surgery LUM University Casamassima Bari Italy"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Valentina Annamaria Genchi",
      "orcid": "0000-0001-8188-9638"
    },
    {
      "affs": [
        0
      ],
      "name": "Celeste Lauriola",
      "orcid": "0000-0001-5318-0875"
    },
    {
      "affs": [
        0
      ],
      "name": "Cristina Caccioppoli"
    },
    {
      "affs": [
        0
      ],
      "name": "Rossella D'Oria",
      "orcid": "0000-0002-9064-2159"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicola Marrano",
      "orcid": "0000-0001-6587-6087"
    },
    {
      "affs": [
        0
      ],
      "name": "Giuseppina Biondi",
      "orcid": "0000-0001-9527-9048"
    },
    {
      "affs": [
        1
      ],
      "name": "Sebastio Perrini",
      "orcid": "0000-0002-2728-7721"
    },
    {
      "affs": [
        0
      ],
      "name": "Annalisa Natalicchio",
      "orcid": "0000-0002-6131-7517"
    },
    {
      "affs": [
        0
      ],
      "name": "Luigi Laviola"
    },
    {
      "affs": [
        0
      ],
      "name": "Francesco Giorgino",
      "orcid": "0000-0001-7372-2678"
    },
    {
      "affs": [
        0
      ],
      "name": "Angelo Cignarelli",
      "orcid": "0000-0001-6477-9031"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6my03xztbr7bh.bib",
  "cited_by": 0,
  "doi": "10.1002/oby.70315",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13716193/",
  "id": "6my03xztbr7bh",
  "issns": [
    "1930-7381",
    "1930-739X"
  ],
  "landing_url": "https://doi.org/10.1002/oby.70315",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "ORIGINAL ARTICLE"
    ],
    [
      "heading",
      "Obesity Biology and Integrated Physiology"
    ],
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "2737|Adipose Tissue and Metabolism"
    ]
  ],
  "openalex": "W7215066566",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/oby.70315",
  "pmcid": "PMC13716193",
  "pmid": "42817866",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6my03xztbr7bh.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:23:14Z",
      "native_id": "PMC13716193",
      "source": "pmc",
      "url": "https://doi.org/10.1002/oby.70315"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Adipose Tissue and Metabolism",
      "subfield": "physiology"
    }
  ],
  "title": "Depot‐Specific Insulin Receptor Isoform Dynamics During Human Adipose‐Derived Stem Cell Adipogenesis",
  "title_text": "Depot‐Specific Insulin Receptor Isoform Dynamics During Human Adipose‐Derived Stem Cell Adipogenesis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:23:14Z",
  "url": "https://research.draconitic.com/paper/6my03xztbr7bh",
  "venue": "Obesity (Silver Spring, Md.)",
  "versions": []
}