{
  "abstract": "Histone H3 lysine 36 dimethylation (H3K36me2) plays a key role in transcriptional elongation and chromatin organization, yet how its deposition is spatially controlled across the genome remains unclear. Here, we identify TOP1 as a critical recruiter of the H3K36me2 methyltransferase NSD2 to gene bodies. Disruption of the TOP1-NSD2 interaction reduces H3K36me2 at gene bodies, impairs RNA polymerase II release, and represses transcription. Remarkably, loss of this recruitment increases H3K36me2 at repetitive sequences, licensing their aberrant transcriptional activation. We show that NSD2 is primarily responsible for depositing H3K36me2 at these loci. We further identify MTA1 as a recruiter of NSD2 to repetitive elements via recognition of the GATC consensus sequence. Together, TOP1 and MTA1 spatially partition NSD2 across the genome, enabling H3K36me2 to serve dual roles in regulating canonical gene expression and acting as an active mark for the transcriptional activation of repetitive sequences.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Hematology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.",
    "Biological Laboratory of Hetao Cooperation Zone, the Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.",
    "Life Sciences Institute, Zhejiang University, Hangzhou, China.",
    "State Key Laboratory of Oncogenes and Related Genes, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.",
    "Institute of Gene Biology, Moscow, Russia.",
    "Life Sciences Institute, Zhejiang University, Hangzhou, China. dfang@zju.edu.cn.",
    "Department of Medical Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. dfang@zju.edu.cn.",
    "Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Hangzhou, China. dfang@zju.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Junqi Jia"
    },
    {
      "affs": [
        2
      ],
      "name": "Yu Liu",
      "orcid": "0009-0008-7722-4324"
    },
    {
      "affs": [
        2
      ],
      "name": "Jiamin Yu"
    },
    {
      "affs": [
        2
      ],
      "name": "Jingwei Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yiting Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuan Fang"
    },
    {
      "affs": [
        3
      ],
      "name": "Ying Yu"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhongxing Sun"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhuoning Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Yin Tang"
    },
    {
      "affs": [
        2
      ],
      "name": "Juejia Shao"
    },
    {
      "affs": [
        2
      ],
      "name": "Yanjun Zhang",
      "orcid": "0000-0002-5695-6488"
    },
    {
      "affs": [
        2
      ],
      "name": "Hai Song",
      "orcid": "0000-0003-3228-6616"
    },
    {
      "affs": [
        4
      ],
      "name": "Sergey V Ulianov"
    },
    {
      "affs": [
        3
      ],
      "name": "Jing Huang",
      "orcid": "0000-0002-0481-8702"
    },
    {
      "affs": [
        5,
        6,
        7
      ],
      "name": "Dong Fang",
      "orcid": "0000-0002-9807-5224"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6mybfw6d9qagk.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77898-y",
  "fulltext_url": "https://europepmc.org/articles/PMC13683359",
  "id": "6mybfw6d9qagk",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://europepmc.org/article/MED/42901929",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Genomics and Chromatin Dynamics"
    ]
  ],
  "openalex": "W7213587955",
  "pdf_url": "https://europepmc.org/articles/PMC13683359?pdf=render",
  "pmcid": "PMC13683359",
  "pmid": "42901929",
  "published": "2026-09-19",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6mybfw6d9qagk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42901929",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42901929"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Genomics and Chromatin Dynamics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Spatial partitioning of NSD2 defines H3K36me2 as an active mark for repetitive sequence activation.",
  "title_text": "Spatial partitioning of NSD2 defines H3K36me2 as an active mark for repetitive sequence activation.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:41Z",
  "url": "https://research.draconitic.com/paper/6mybfw6d9qagk",
  "venue": "Nature communications",
  "versions": []
}