{
  "abstract": "Inflammatory bowel disease (IBD) involves dysregulated mucosal immunity, oxidative stress, impaired epithelial barrier integrity and gut microbial dysbiosis. We evaluated a two-strain formulation of Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13 in DNBS-induced colitis. Twenty-four male Sprague Dawley rats were randomised into three groups (n = 8): control, acute colitis (AC; DNBS 35 mg/0.25 mL per rat rectally on days 0 and 1), and probiotic treated (RP; 3.6 × 109 CFU daily by gavage, days −3 to 6). DNBS raised Ameho histological scores, serum TNF-α, colonic MDA and MPO positivity and lowered IL-10, occludin and claudin-1 (all p < 0.05). Relative to AC, probiotic-treated rats showed lower TNF-α (p = 0.002) and MPO positivity (p < 0.001) and higher IL-10 (p = 0.001), occludin (p = 0.014) and claudin-1 (p = 0.001). Colonic MDA and Ameho scores were numerically lower in the RP group but these differences did not reach statistical significance (p = 0.085 and p = 0.310). Serum IL-17A was unchanged. Microbial community composition differed among groups (PERMANOVA pseudo-F = 5.798, R2 = 0.356, p = 0.0001), with the probiotic-associated community distinct from both control and colitis communities; distance-based analysis detected no convergence toward the control community. The formulation attenuated inflammatory and barrier outcomes and altered microbiota composition without restoring it.",
  "abstract_shown": true,
  "affiliations": [
    "Center of Excellence in Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases, Chulalongkorn University, Bangkok 10330, Thailand",
    "Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand",
    "Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand",
    "Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand",
    "Center of Excellence in Hepatitis and Liver Cancer, Chulalongkorn University, Bangkok 10330, Thailand",
    "Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Pakkapon Rattanachaisit"
    },
    {
      "affs": [
        2
      ],
      "name": "Nathawadee Lerttanatum"
    },
    {
      "affs": [
        3
      ],
      "name": "Somying Tumwasorn"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Natthaya Chuaypen"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Thasinas Dissayabutra"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Duangporn Werawatganon",
      "orcid": "0000-0001-8847-9690"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6n774chjxmpve.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219961",
  "fulltext_url": null,
  "id": "6n774chjxmpve",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9961",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/6n774chjxmpve.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T00:21:27Z",
      "native_id": "10.3390/ijms27219961",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9961"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Effects of a Two-Strain Lactobacillus Formulation (Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13) on Inflammatory and Oxidative Markers, Epithelial Junction Proteins, and Fecal Microbiota in Rats with DNBS-Induced Colitis",
  "title_text": "Effects of a Two-Strain Lactobacillus Formulation (Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13) on Inflammatory and Oxidative Markers, Epithelial Junction Proteins, and Fecal Microbiota in Rats with DNBS-Induced Colitis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T00:21:27Z",
  "url": "https://research.draconitic.com/paper/6n774chjxmpve",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}