{
  "abstract": "Bone repair is a complex regenerative process relying on skeletal stem/progenitor cells (SSPCs) recruited predominantly from the periosteum. Activation and differentiation of periosteal SSPCs occurs in an heterogeneous environment, rising the need of using single cell/nucleus transcriptomics in order to decipher the response of the periosteum to injury. Yet, enzymatic cell dissociation can induce a stress response affecting the transcriptome and lead to overrepresentation of certain cell types (i.e. immune and endothelial cells) and low coverage of other cell types of interest. To counteract these limitations, we optimized a protocol to isolate nuclei directly from the intact periosteum and from the fracture callus to perform single-nucleus RNA sequencing (snRNAseq). This protocol is adapted for fresh murine periosteum, fracture callus and for frozen human periosteum. Nuclei are isolated using mechanical extraction combined with fluorescence-based nuclei sorting to obtain high-quality nucleus suspension. This protocol allows to capture the full diversity of cell types in the periosteum and the fracture environment to better reflect the in vivo tissue composition.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Simon Perrin"
    },
    {
      "name": "Cassandre Goachet"
    },
    {
      "name": "Maria Ethel"
    },
    {
      "name": "Yasmine Hachemi"
    },
    {
      "name": "Céline Colnot"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6p70ab5x3vxyg.bib",
  "cited_by": 0,
  "doi": "10.21769/bioprotoc.5211",
  "fulltext_url": null,
  "id": "6p70ab5x3vxyg",
  "issns": [
    "2331-8325"
  ],
  "landing_url": "https://u-pec.hal.science/hal-05772616v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
    ]
  ],
  "openalex": "W4406709811",
  "pdf_url": "https://u-pec.hal.science/hal-05772616/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Bio-protocol LCC",
  "ris": "https://research.draconitic.com/paper/6p70ab5x3vxyg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:45Z",
      "native_id": "hal-05772616",
      "source": "hal",
      "url": "https://u-pec.hal.science/hal-05772616v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Single-cell and spatial transcriptomics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Nuclei Isolation From Murine and Human Periosteum For Transcriptomic Analyses",
  "title_text": "Nuclei Isolation From Murine and Human Periosteum For Transcriptomic Analyses",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:45Z",
  "url": "https://research.draconitic.com/paper/6p70ab5x3vxyg",
  "venue": "Bio-protocol",
  "versions": []
}