{
  "abstract": "Microbial fermentation is an effective approach to improving the nutritional value of cottonseed hulls and alleviating feed resource shortages. However, the interaction mechanisms between Bacillus and Lactobacillus within fermentation systems remain unclear. In this study, a composite Bacillus group (Group B), a composite Lactobacillus group (Group R), and a natural fermentation control group (Group C) were established for a 30-day solid-state fermentation experiment. Physicochemical parameters were measured at multiple time points, and 16 S rRNA amplicon sequencing was performed to systematically analyze fermentation quality changes and microbial community succession. It was found that, compared with Groups R and C, Group B exhibited superior performance in degrading antinutritional factors and macromolecular substrates. The degradation rate of free gossypol reached 80.52%, while crude fiber content was reduced by 14.98%. Microbial community structure analysis revealed significant differences in composition among the treatment groups. Functional prediction analysis suggested that Group B was enriched in pathways associated with carbohydrate and amino acid metabolism, whereas Group R showed higher representation of pathways related to energy metabolism and cofactor and vitamin biosynthesis. Network analysis demonstrated that the introduction of Bacillus increased microbial network complexity while reducing cooperative interactions, indicating a strong competitive effect during fermentation. LEfSe analysis further revealed that different treatments significantly shaped distinct microbial biomarkers: Group B was enriched in Acinetobacter, Brevibacterium, and Faecalibacterium; Group R was dominated by Acidobacterium and Megasphaera; and Group C was dominated by Enterobacter and Staphylococcus. Correlation analysis revealed that 19 bacterial genera were significantly associated with nutrient transformation indicators, suggesting a strong coupling between microbial structure and functional processes. In summary, Bacillus and Lactobacillus exhibit a functional division of labor, characterized by early-stage degradation and later-stage stabilization during cottonseed hull fermentation. This study provides a theoretical foundation for optimizing microbial consortia and promoting the high-value utilization of agricultural waste.",
  "abstract_shown": true,
  "affiliations": [
    "Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.",
    "Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830017, China.",
    "Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China. cuwedo@163.com.",
    "Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China. hmde_092@outlook.com."
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Kunyi Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Yang Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Xin Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Huaibing Yao"
    },
    {
      "affs": [
        0
      ],
      "name": "Gulinigaer Aiyisirehong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Nuerbiya Aibaidula"
    },
    {
      "affs": [
        2
      ],
      "name": "Weidong Cui"
    },
    {
      "affs": [
        3
      ],
      "name": "Min Hou"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6r0gg5hynbmmw.bib",
  "cited_by": 0,
  "doi": "10.1186/s12866-026-05090-9",
  "fulltext_url": "https://europepmc.org/articles/PMC13726030",
  "id": "6r0gg5hynbmmw",
  "issns": [
    "1471-2180"
  ],
  "landing_url": "https://europepmc.org/article/MED/42967977",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1103|Animal Nutrition and Physiology"
    ]
  ],
  "openalex": "W7172390330",
  "pdf_url": "https://europepmc.org/articles/PMC13726030?pdf=render",
  "pmcid": "PMC13726030",
  "pmid": "42967977",
  "published": "2026-08-05",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/6r0gg5hynbmmw.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:54Z",
      "native_id": "MED:42967977",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42967977"
    },
    {
      "first_seen": "2026-11-05T12:22:27Z",
      "native_id": "PMC13726030",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12866-026-05090-9"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Nutrition and Physiology",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Comparative evaluation of Bacillus and Lactobacillus in cottonseed hull fermentation.",
  "title_text": "Comparative evaluation of Bacillus and Lactobacillus in cottonseed hull fermentation.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:22:05Z",
  "url": "https://research.draconitic.com/paper/6r0gg5hynbmmw",
  "venue": "BMC microbiology",
  "versions": []
}