{
  "abstract": "Background: Donor organ shortage has led to increased reliance on extended-criteria donor (ECD) livers for transplantation. Normothermic machine perfusion (NMP) has led to a paradigm shift in organ preservation from cold storage to full ex-situ metabolic support. This study investigated ECD liver metabolic profiling to elucidate NMP molecular signatures and whether signatures were predictive of graft viability and clinical outcome.\n\nMethods: Thirty-one livers underwent NMP for a minimum of four hours and 23 livers fulfilled NMP viability criteria. Perfusate from graft perfusion commencement to 4-h time point were collected at 0.25, 2 and 4 h. Samples underwent untargeted metabolomics analysis using Ultra High Performance Liquid Chromatography-Mass Spectrometry. Univariate statistical analyses revealed dynamic changes in metabolic profiles across the perfusion period and differences in water-soluble and lipid-based metabolites between viable and non-viable livers.\n\nResults: Pathway enrichment analysis highlights pathways relating to amino acid and energy metabolism differing between viable and non-viable livers. Metabolic changes are not observed for ECD livers based on post-transplant clinical outcome.\n\nConclusions: This study revealed metabolic signatures of ECD livers that may define metabolic differences related to graft viability which can be targeted for improved viability.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 27,
  "authors": [
    {
      "name": "Joseph A. Attard",
      "orcid": "0000-0001-5770-2896"
    },
    {
      "name": "Richard Laing"
    },
    {
      "name": "Lorraine Wallace"
    },
    {
      "name": "Lukas Najdekr"
    },
    {
      "name": "Gavin R. Lloyd"
    },
    {
      "name": "Andris Jankevics"
    },
    {
      "name": "Amanda Kirkham"
    },
    {
      "name": "Yuri L. Boteon",
      "orcid": "0000-0002-1709-9284"
    },
    {
      "name": "Darren Barton",
      "orcid": "0000-0003-0106-5861"
    },
    {
      "name": "Stuart Curbishley"
    },
    {
      "name": "Manpreet Wilkhu"
    },
    {
      "name": "Desley A. H. Neil",
      "orcid": "0000-0001-9800-6811"
    },
    {
      "name": "Paolo Muiesan"
    },
    {
      "name": "John R. Isaac"
    },
    {
      "name": "Keith J. Roberts"
    },
    {
      "name": "Manuel Abradelo"
    },
    {
      "name": "Andrea Schlegel"
    },
    {
      "name": "James Ferguson"
    },
    {
      "name": "Hentie Cilliers"
    },
    {
      "name": "Chris Morris",
      "orcid": "0000-0001-9102-6668"
    },
    {
      "name": "Peter J. Friend",
      "orcid": "0000-0003-0841-9685"
    },
    {
      "name": "Christina Yap"
    },
    {
      "name": "Hynek Mergental",
      "orcid": "0000-0001-5480-9380"
    },
    {
      "name": "Darius F. Mirza",
      "orcid": "0000-0002-7531-9089"
    },
    {
      "name": "M. Thamara P. R. Perera"
    },
    {
      "name": "Warwick B. Dunn",
      "orcid": "0000-0001-6924-0027"
    },
    {
      "name": "Simon C. Afford"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6r5j5gtq1q5rj.bib",
  "cited_by": 0,
  "doi": "10.1038/s43856-025-00737-6",
  "fulltext_url": null,
  "id": "6r5j5gtq1q5rj",
  "issns": [
    "2730-664X"
  ],
  "landing_url": "https://www.nature.com/articles/s43856-025-00737-6",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2746|Organ Transplantation Techniques and Outcomes"
    ]
  ],
  "openalex": "W7221614357",
  "pdf_url": "https://www.nature.com/articles/s43856-025-00737-6_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-13",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/6r5j5gtq1q5rj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:53Z",
      "native_id": "10.1038/s43856-025-00737-6",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s43856-025-00737-6"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2746|Organ Transplantation Techniques and Outcomes",
      "subfield": "surgery"
    }
  ],
  "title": "Molecular fingerprints linked to graft viability in extended criteria donor livers during normothermic machine perfusion",
  "title_text": "Molecular fingerprints linked to graft viability in extended criteria donor livers during normothermic machine perfusion",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:53Z",
  "url": "https://research.draconitic.com/paper/6r5j5gtq1q5rj",
  "venue": "Communications Medicine",
  "versions": []
}