{
  "abstract": "Three novel Cu(II) complexes with 3,4-dimethoxybenzylidene-substituted thiosemicarbazones were synthesized and evaluated for anticancer, antibacterial, and antioxidant activities. Our recent structure-activity relationship studies showed that Cu(II) complexes containing 3,4-dimethoxybenzylidene-substituted ligands can display potent anticancer activity, reaching single-digit IC 50 values, which prompted further investigation of this structural motif. The complexes examined here showed moderate antibacterial activity and distinct antioxidant profiles in the ABTS, DPPH, and ORAC-FL assays. Among them, the morpholine-containing complex Cu(dm-TSC-morph) 2 showed the most favorable anticancer selectivity, retaining low-micromolar activity against SK-MEL-28 and A-375 melanoma cells (IC 50 = 6.58 ± 0.71 and 9.20 ± 1.52 μM, respectively) while exhibiting lower toxicity toward BJ, HUVEC, and HEK-293 cells, with selectivity indices up to 4.88. Studies of mechanism of action revealed that Cu(dm-TSC-morph) 2 induced intracellular oxidative stress in melanoma cells. These findings identify the morpholine-containing derivative as the most promising compound in the analyzed series and support further development of selective Cu(II) thiosemicarbazone complexes.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Bartłomiej Rogalewicz",
      "orcid": "0000-0002-9899-6402"
    },
    {
      "name": "Marcin Świątkowski"
    },
    {
      "name": "Magdalena Iwan"
    },
    {
      "name": "Joanna Kubik"
    },
    {
      "name": "Agnieszka Korga-Plewko"
    },
    {
      "name": "Monika Pitucha"
    },
    {
      "name": "Emil Borecki"
    },
    {
      "name": "Tomasz Boruta"
    },
    {
      "name": "Sylwia Ścieszka"
    },
    {
      "name": "Edyta Kordialik-Bogacka"
    },
    {
      "name": "Aleksandra Lis"
    },
    {
      "name": "Paweł Szymański"
    },
    {
      "name": "Anna Gajda"
    },
    {
      "name": "Agnieszka Czylkowska"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6r9xx9x9jvfjr.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009970/v1",
  "fulltext_url": null,
  "id": "6r9xx9x9jvfjr",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009970/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Metal complexes synthesis and properties"
    ]
  ],
  "openalex": "W7220740438",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15009970/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-06",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/6r9xx9x9jvfjr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:22Z",
      "native_id": "10.26434/chemrxiv.15009970",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009970/v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Metal complexes synthesis and properties",
      "subfield": "oncology"
    }
  ],
  "title": "Cu(II) complexes based on morpholine-substituted thiosemicarbazones show reduced toxicity in normal cells",
  "title_text": "Cu(II) complexes based on morpholine-substituted thiosemicarbazones show reduced toxicity in normal cells",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:22Z",
  "url": "https://research.draconitic.com/paper/6r9xx9x9jvfjr",
  "venue": "ChemRxiv",
  "versions": []
}