{
  "abstract": "BACKGROUND AND OBJECTIVES: Accurate detection of staphylococcal beta-lactamase remains a diagnostic challenge, despite its importance for guiding antibiotic therapy, particularly in severe infections, such as endocarditis and bacteraemia. Currently, no available method reliably detects beta-lactamase activity in all staphylococcal species. Here, we evaluated a novel fluorogenic biotracer, 352600-C (MOLSID, Lyon, France), designed to detect beta-lactamase activity in staphylococci. METHODS: The 352600-C biotracer was incorporated into Mueller-Hinton agar plates onto which staphylococcal strains were subcultured at 37°C. Colony fluorescence was assessed at 4, 6 and 24 h using a 365 nm UV lamp. The assay was performed on a large collection of blaZ-positive (n = 124, including BlaZ types A, B, C and D) and blaZ-negative (n = 69) staphylococci from 18 species (Staphylococcus aureus, n = 100; S. non-aureus, n = 93). Molecular detection of the blaZ gene using WGS served as the gold standard. Phenotypic susceptibility to penicillins was also tested using EUCAST recommendations. RESULTS: After 4, 6 and 24 h of incubation, the assay showed sensitivities of 92.2%, 96.9% and 100% for S. aureus and 50.0%, 88.3% and 100% for non-aureus staphylococci, respectively. None of the 69 blaZ-negative strains produced fluorescent colonies (specificity 100%). Notably, the assay detected all BlaZ types. Penicillin disc diffusion showed 98.4% sensitivity and 77.8% specificity for MSSA. CONCLUSIONS: This novel assay for the detection of staphylococcal beta-lactamase demonstrated simplicity, rapidity and excellent accuracy after 24 h incubation (100% sensitivity and specificity) across a genetically diverse and representative collection of clinical isolates, making it a valuable tool for routine diagnostics and antimicrobial stewardship.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Céline Dupieux"
    },
    {
      "name": "Patricia Martins Simoes"
    },
    {
      "name": "Benjamin Youenou"
    },
    {
      "name": "François Vandenesch"
    },
    {
      "name": "Anne Tristan"
    },
    {
      "name": "Frédéric Laurent"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6rkqm6c7sw6bn.bib",
  "cited_by": 0,
  "doi": "10.1093/jac/dkag268",
  "fulltext_url": null,
  "id": "6rkqm6c7sw6bn",
  "issns": [
    "0305-7453",
    "1460-2091"
  ],
  "landing_url": "https://hal.science/hal-05781324v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2725|Antimicrobial Resistance in Staphylococcus"
    ]
  ],
  "openalex": "W7203455275",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-04",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/6rkqm6c7sw6bn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "hal-05781324",
      "source": "hal",
      "url": "https://hal.science/hal-05781324v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Antimicrobial Resistance in Staphylococcus",
      "subfield": "infectious-disease"
    }
  ],
  "title": "A fluorogenic biotracer for reliable detection of staphylococcal beta-lactamase: towards improved precision therapy in severe MSSA infections",
  "title_text": "A fluorogenic biotracer for reliable detection of staphylococcal beta-lactamase: towards improved precision therapy in severe MSSA infections",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:49Z",
  "url": "https://research.draconitic.com/paper/6rkqm6c7sw6bn",
  "venue": "Journal of Antimicrobial Chemotherapy",
  "versions": []
}